1. MyStem® Autograft Preparation Kit
Regenerative Treatments made easy

2. The People Pier Ivona, IFATS Member, WOSIAM Member,
Regenerative Medicine Expert, Entrepreneur
Mr. Ivona has a multi year experience in Regenerative treatments and MedDev industry,
His experience leads the team who developed MyStem technology

3. The Science - Stromal tissue -
Scientific Definition:
The connective tissue framework of anorgan, a gland, or other structure, as distinguished from the  tissues performing the special function of the organ or part.
Stroma is a broadly used term for the loose connective tissue that contains mesenchymal stem cells and other cells like fibroblasts, macrophages, adipocytes, mast cells and leukocytes.
Source:
The American Heritage® Stedman's Medical Dictionary

4. The Science - Stromal tissue – Why Adipose stromal tissue a Regenerative fraction source?
Adipose tissue as a Stromal Tissue source provides regenerative components in high ratio

5. The Science - Stromal tissue –
Adipose tissue as a Stromal Tissue source provides regenerative components in great numbers (Stromal Vascular Fraction-SVF)

6. The Stromal Vascular Fraction (SVF)
Derived from Blood Vessels in adipose (fat) stromal tissue
Capillaries are primary source (see inset below)
Same-day clinical procedure
Easy to harvest
High cell counts
Blood vessels / Capillaries
Adipose (fat) cells

7. The Stromal Vascular Fraction (SVF)
The isolated SVF contains:
Adult Stem Cells
Endothelial Cells
Fibroblast Cells
Pericytes
Red Blood Cells
White Blood Cells
… and much more

8. ADSCs are Growth Factors and Cytokines factories
Flt-3 ligand,
granulocyte colony stimulating factor (G-CSF)
granulocyte/macrophage colony stimulating factor (GM-CSF)
macrophage colony stimulating factor (M-CSF)
interleukin-6 (IL-6)
interleukin-7 (IL-7)
interleukin-8 (IL-8)
interleukin-11 (IL-11)
 interleukin-12 (IL-12)
leukemia inhibitory factor (LIF)
tumor necrosis factor-alpha (TNF-α)
Growth Factors 
epidermal growth factor (EGF)
vascular endothelial growth factor (VEGF)
basic fibroblast growth factor (bFGF)
 keratinocyte growth factor (KGF)
platelet-derived growth factor (PDGF),
 hepatocyte growth factor (HGF)
transforming growth factor-beta (TGF-β)
insulin-like growth factor (IGF)
 brain-derived neurotrophic factor( BDNF)

9. The Technology - the MyStem concept -
Our Vision: Providing Regenerative fraction at the point-of-care

10. MYSTEM® Process - Step by Step – (can be repeated several times for the same patient)

11. up to 10x volume reduction
1111
MYSTEM® SVF isolation Process (the process can be repeated several times for the same patient)
1.Tissue Harvesting
2.Fractioning
Liquid Fraction
Fat Fraction
3.Cells Separation
7.1 millions cells / harvested ml
4.Cells Concentration
up to 10x volume reduction

12. The Technology - the MyStem device -
Single-use Medical Device
One-step Procedure
Process time 15 min
Nucleated Cells / ml
Up to 10x Concentration
International Patent

13. Clinical use of MyStem technology
Demonstrated effects in:
Bone tissue repair (Published Study)
Osteoarthritis (Case Report)
Intervertebral Disc disease (Published Study)
Tendon injury (Case Report)
Severe Burns (Case Report)
Soft tissue augmentation (Case Report)
Wound healing (Case Report)
Diabetic Foot Syndrome (Case Report)
Crohns (Case Report)
Fistulas (Case Report)

14. MyStem Factsheet MyStem Authorizations Clinical Evidence CE Mark
SFDA (Saudi FDA)
TGA (Australia)
ANVISA (Brazil)
Clinical Evidence
over 1200 clinical cases done

15. Together.....
...helping people...
..with great results..

16. Thank you