1. WITH MCS+ AND TRIMA SEPARATORS.
EVALUATION OF PLATELETS CONCENTRATES AND RED BLOOD CELLS CONCENTRATES PRODUCTION
WITH MCS+ AND TRIMA SEPARATORS.
Rapaille A, Gillet P, Sondag-Thull D.
Blood Transfusion Centre, Belgian Red Cross, Liège, Belgium.
BACKGROUND.
Multi-component apheresis technology has become a routine method in order to obtain several highly standardised products, like leukoreduced platelets concentrates (PC), plasma and leukoreduced red blood cells concentrates (RBCC).
We evaluated different collection protocols to obtain PC, RBCC and plasma.
METHODS.
Protocol LDP LDP LDPRBC LDPRBC RBCP+ SDR
Separator MCS Trima MCS Trima MCS MCS
Products PC+Plasma PC+ Plasma PC+RBCCC PC+RBCC RBCC+Plasma RBCC+RBCC
Filtration process Pall LRP6 LRS Pall LRP6 LRS Pall RC1H Hemasure Hight-crit Pall RC1H Pall BPF4H
Max Time 90 min. 90 min.
Product parameters n.d. 3.5/ Plat n.d. 3.5/ Plat
Vol. RBC before filtration (ml)
Vol. RBC after filtration (ml)
Hgb after filtration (gr.)
Donation times were recorded. Samplings were taken day of collection. Cells numeration, haemoglobin, hematocrit were performed on Cell-dyn 3000 (Abbott).
Residual White Blood Cells (WBC) were counted with a microscope in Nageotte chambers after dilution in 1 to 5 in Turk’s solution for PC and in 1 to 10 in Plaxan solution for RBCC.
FINDING.
Results in mean and SD after leukoreduction of products.
Protocol LPD LDP LDPRBC LDPRBC RBCP+ SDR
number
Time (min) 84+/ / / / /-4 28+/-3
Vol. PC (ml) 325+/ / / /-52
Plat (1011) 5.2+/ / / /-1.5
WBC (105)
Vol. RBCC (ml) / / / /-12
Hgb (gr.) / / / /-2.8
Hc (%) / / / /-1.1
Vol. RBC (ml) / / / /-8
WBC (105)
Vol. Plasma (ml) 368+/ / /-38
CONCLUSION.
Multi-component collection by apheresis technique is a feasible method to obtain RBCCs and PCs in high production consistency and quality in respect of donor safety (Poster 535: Are MCS+ and Trima safe and convenient for donors?). All PCs (except two collected with the LDP protocol, one with MCS and the other with Trima separator, for residual WBC) and all RBCCs meet also the European guideline recommendations. .