1. Buffy Coat Method Platelet Poor Plasma Step 1: Whole Blood is collected into a top and bottom pack and is centrifuged (hard spin) to produce platelet poor plasma, red blood cells and a buffy coat Buffy Coat Layer Red Cell Concentrate Red Cell Additive Images courtesy of Canadian Blood Services – Hospital Education Presentation 2004

2. Step 2 Extraction Plasma Red blood cell Buffy Coat Buffy Coat Method cont.

3. Step 3: Pooled Buffy Coats are first pooled, then sterile docked to the platelet storage bags, followed by centrifugation at low speed Buffy Coat Method cont.

4. Buffy Coats sterile docked together Plasma Pooling (Train Method) Buffy Coat Method cont. Platelet storage bag sterile docked to the Buff Coat “train”

5. Buffy Coat Pool is loaded into buffy coat bag Supernatant (PRP) pressed through LR filter into storage bag Buffy Coat Method cont

6. Pooled Platelet Concentrate, LR is Similar in physical size & dosage to an apheresis platelet concentrate Buffy Coat Method cont.