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Dietary nitrite induces nitrosation of the gastric mucosa: the protective action of the mucus and the modulatory effect of red wine  Cassilda Pereira,

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Presentation on theme: "Dietary nitrite induces nitrosation of the gastric mucosa: the protective action of the mucus and the modulatory effect of red wine  Cassilda Pereira,"— Presentation transcript:

1 Dietary nitrite induces nitrosation of the gastric mucosa: the protective action of the mucus and the modulatory effect of red wine  Cassilda Pereira, Rui M. Barbosa, João Laranjinha  Journal of Nutritional Biochemistry  Volume 26, Issue 5, Pages (May 2015) DOI: /j.jnutbio Copyright © 2015 Elsevier Inc. Terms and Conditions

2 Fig. 1 Typical nitric oxide chemiluminescent signals obtained after duplicate injections (100μl) of nitrite standard solutions (from 50 nM to 10μM) into the reaction mixture. Inset: Standard calibration curve from the same experiment obtained by plotting the geometric mean±S.E.M. of the areas under curve (AUC) of individual peaks vs. nitrite concentration. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

3 Fig. 2 Nitrosation of mucin by nitrite under simulated gastric conditions. The values are expressed as mean±S.E.M., n=2–3. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

4 Fig. 3 Pattern of gastric mucus nitrosation upon nitrite exposure under simulated gastric conditions ex vivo in a whole stomach model (left) and the corresponding nitric oxide formation in vitro (right) in a similar medium. Values are mean±S.E.M. (n=3–7). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

5 Fig. 4 Gastric mucus nitrosation pattern upon exposure to nitrite and red wine (RW) mixtures under simulated gastric conditions (left). Right, in vitro nitric oxide formation from acidic nitrite from the corresponding mixtures. Values are mean±S.E.M. (n=3–7), *P<.05. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

6 Fig. 5 Pattern of gastric mucosa nitrosation upon nitrite exposure under simulated gastric conditions ex vivo in a whole stomach model (left) and the corresponding nitric oxide formation in vitro (right) in a similar medium. Values are mean±S.E.M. (n=3–4). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

7 Fig. 6 Effect of red wine (RW) on the nitrosation pattern of gastric mucosa challenged with nitrite under simulated gastric conditions (left). Right, in vitro nitric oxide formation from acidic nitrite from the corresponding mixtures. Values are mean±S.E.M. (n=3), *P<.05. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

8 Fig. 7 Effect of mucus gel removal on the nitrosation of the gastric mucosa exposed to nitrite (1mM). Mucus was removed by scrapping or by pre-incubation of the mucosa with 2 % N-acetyl-cysteine (NAC). Values are mean±S.E.M. (n=3), *P<.05, relative to control. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2015 Elsevier Inc. Terms and Conditions

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