1. By Kurt Osther, MD Micael Haugegaard, MD Hanne Everland, PhD
MPEG-PLGA Aseed Scaffold Enhanced Microfracturing of Articular Cartilage Defects in knees and ankles (Aseed-SEMT) A suggested future cartilage repair technique By
Kurt Osther, MD
Micael Haugegaard, MD
Hanne Everland, PhD

2. Cartilage Biopsy from 29 patients, 12 months after ACI with Periost minus/plus chondrocyte Implantation (2006)

3. Colonization from Explants in a Cell Culture flask
Mesenchymal
Cell colonies
Explants

4. The Unique Explant culture technology for obtaining young chondroblasts at day 7 or older starts to adhere to the bottom of the tissue culture flasks,
Chondroblast migration 7 days post-processing
Chondroblast migration 7 days post-processing; Toluidine Blue Stain
Cell Explants are cultured in DMEM/F12 with Proprietary Autologous
Serum Fraction, Kurt Osther, Proprietary information!

5. Coloplast’s Aseed™ scaffold Coloplast Patent
Scanning Electron Microscopy
of Aseed™ scaffold.
>90% porous & hydrophilic
absorbs quickly viscous fluid (BM),
differentiate retained cells to
Chondrogene cells (see histology)
Aseed™ (synthetic) Scaffold absorbed after ~ 4 months
(beta irradiated)

6. Culturing “in vitro” on Aseed shows migration into the scaffold after 3 days
The red bars correspond to 200 μm
On day 1 (left panel), the cells were
distributed in a layer
on the top of the scaffold.
On day 3 (right panel), the cells
had migrated into the scaffold..

7. Chondrogenic Characteristics on Aseed™
Safranin O
Toluidine Blue
Histology of Progenitor cells in Aseed, 7 day
MPPEG-PLGA
Control
SOX9
Collagen Type II
Gene expression
Progenitor cells in 7 day culture
Aggrecan
Collagen Type II
Immunohistochemistry
Chondroblasts after 4 weeks culture

8. Operations Goats, Research Center Foulum, Denmark

9. Histology on the Goats EMTPY (NoTreatment) Microfracture
Progenitors in fibrin
Aseed ~ progenitors

10. Improvement in cartilage parameters
Macroscopic Score p<0.05
Histologic Appearance
ICRS Score:
Aseed/cells: 8-9 (normal 10)
Tissue fill: close 100
(norm: ~100)
Empty microfr. fibrin/cells Aseed/cells
Empty microfr. fibrin/cells Aseed/cells
O’Driscoll’s Score p<0.001
Pinada Score p<0.001
O’Driscoll Score:
Aseed/cells:20-23 (norm.25)
Pinada Score:
Aseed/cells: 2-3 (norm. 0)
(histomorphometry)
(semiquantitative scale for 
grading the natural 
healing process)
Empty microfr. fibrin/cells Aseed/cells
Empty microfr. fibrin/cells Aseed/cells

11. The proposed Aseed™-SEMT Process: 1. cleaning the defect, 2
The proposed Aseed™-SEMT Process: 1. cleaning the defect, 2.perform microfracture with awls, 3. quickly, glue Aseed scaffold to defect (the SEMT technique) op
Make tiny fractures with awl into subchondral
bone, cut sterile Aseed scaffold to exact size.
Add fibrin glue, & press Aseed cover over
microfractured area
Clean cartilage defect and cut
to fresh cartilage edges,

12. Thank You Kurt Osther, MD Pathologist Osther Constulting
The Goat study was done by Professor Martin Lind et al, DK (Lind M, Larsen A, Clausen C, Osther K, Everland H. Cartilage repair with chondrocytes in fibrin hydrogel and MPEG polylactide scaffold: an in vivo study in goats. Knee Surg Sports Traum Arthr.2008,16:690)
Thank You
Kurt Osther, MD
Pathologist
Osther Constulting
Scottsdale, Arizona
Micael Haugegaard, MD
Orthopaedic Surgeon
Gildhoj Private Hospital
Copenhagen, Denmark
Hanne Everland, PhD
Director, GRD,
Coloplast A/S
Humlebaek, Denmark
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