1. Biochemistry Free For All
Transcription
Biochemistry Free For All

2. Structure

3. Central Dogma
Template Strand
Coding Strand

4. RNA Functions

5. RNA Building Blocks

6. Eukaryotic Process
Structure

8. Subunits (Prokaryotes)
RNA Polymerase
Subunits (Prokaryotes)
α (2) β β’ ω σ

9. Non-template Strand
Transcription
Template Strand
RNA
Polymerase
RNA

10. Prokaryotic Control Sequences

11. Prokaryotic RNA Polymerase (RNAP)

12. Structure

13. Transcription Initiation and Elongation
Rifampicin Blocks
Elongation
Transcription Bubble

14. Transcription Elongation

15. Transcription Termination

16. Structure

17. Termination
Rho Factor Termination - Rho Protein “Climbs” RNA Being Synthesized and Removes RNA Polymerase from DNA
Factor Independent - Sequence in RNA Causes RNA Polymerase to Fall Off of DNA

18. Chromosome Structure

20. Eukaryotic Control Sequences & Structure

21. Eukaryotic Transcription Complex

22. Transcription Complex Assembly

24. Eukaryotic RNA Polymerase Inhibition
α-amanitin

25. Eukaryotic mRNA 5’ Cap

26. Eukaryotic mRNA 5’ Cap Roles for the 5’ Cap in Eukaryotes
Nuclease Protection / mRNA Stability
Role in Translation

27. Eukaryotic RNA Maturation

28. Splicing

29. Splicing

30. Pyrimidine Rich Sequence
Splicing
GU Sequence
AG Sequence
1. U1 Binding
2. U2 Binding

31. snRNPs - Contain Proteins and snRNAs.
Splicing
Some snRNAs are Complementary to Sequences in Intron and Help to Assemble the Structure

32. Splicing Mechanism

33. Alternative Splicing

34. Alternative Polyadenylation

35. RNA Editing

36. Structure

37. CCA End
tRNA Structure

38. Synthesis of Pseudouridine from Uridine
tRNA
Synthesis of Pseudouridine from Uridine

39. tRNA and rRNA Processing in Eukaryotes

40. Structure

41. Structure

42. Bacteria Eukaryotes Structure
5S rRNA (in large ribosomal subunit - structural support)
16S rRNA (in small ribosomal subunit - aligns mRNA for translation)
23S rRNA (in large ribosomal subunit - catalyzes peptide bond formation
Eukaryotes
5S rRNA (large ribosomal subunit - structural support)
5.8S rRNA (large ribosomal subunit - translocation)
18S rRNA (small ribosomal subunit - functions in translation)
28S rRNA (large ribosomal subunit - peptide bond formation)

43. Structure RNA Functions Synthesis of Proteins (tRNA, rRNA, mRNA)
Primary Genetic Material (RNA Viruses)
Catalysis of Reactions (Ribozymes)
Regulation of Gene Expression (miRNA & siRNA)
Processing of Other RNAs (snRNAs & snoRNAs),
RNA Structures
Ribonucleotides - A,U,C,G
Single Stranded/Self Pairing
G-U Base Pairs

44. Structure