Presentation on theme: "1. Important Features a. DNA contains genetic template" for proteins."— Presentation transcript:
1 1. Important Features a. DNA contains genetic template" for proteins. b. DNA is found in the nucleusc. Protein synthesis occurs in the cytoplasm - ribosome.d. "Genetic information" must be transferred to the cytoplasm where proteins are synthesized.
2 2. Processes of Protein Synthesis a. Transcription - genetic template for a protein is copied and carried out to the cytoplasmb. Translation - template serves as a series of codes for the amino acid sequence of the protein
3 Cells Use RNA to Make Protein The RNA Players – mRNA, rRNA, tRNADuring polypeptide synthesis, ribosomal RNA (rRNA) is the site of polypeptide assembly.Transfer RNA (tRNA) transports and positions amino acids.Messenger RNA (mRNA) directs which amino acids are assembled into polypeptides.Central DogmaDNA RNA Protein
5 Gene Expression Transcription – in the nucleus (if you have one) DNA sequence is transcribed into RNA sequenceinitiated when RNA polymerase binds to promoter binding sitemoves along DNA strand and adds corresponding complementary RNA nucleotidedisengages at stop signal
6 3. Steps of Transcription a. DNA unwindsb. One side of DNA "codes for a protein"c. Genetic code of DNA is a triplet code of 3 nucleotides or basesd. Each triplet is specific for the coding of a single amino acide. Sequence of triplet codes on DNA will specify the amino acid sequence on the proteinf. Major step is the synthesis of the coded "messenger" molecule – mRNAg. mRNA is "transcribed" from DNA by complementary base pairing (mRNA has no thymine, which is replaced by uracil)h. mRNA passes out to cytoplasm to the ribosome
7 Transcription RNA polymerase only one of two DNA strands (template) is transcribednon-transcribed strand is termed coding strand - same as RNA (except T’s are U’s)In both bacteria and eukaryotes, the polymerase adds ribonucleotides to the growing 3’ end of an RNA chain.synthesis proceeds in 5’3’ direction
9 Transcription Promoter Initiation Transcription starts at RNA polymerase binding sites called promoters on DNA template strand.InitiationOther eukaryotic factors bind, assembling a transcription complex.RNA polymerase begins to unwind DNA helix.
10 Transcription Elongation Termination Transcription bubble moves down DNA at constant rate leaving growing RNA strands protruding from the bubble.TerminationStop sequences at the end of the gene cause phosphodiester bond formation to cease, transcription bubble to dissociate, and RNA polymerase to release DNA.
11 TranscriptionEukaryotic transcription differs from prokaryotic transcription:three RNA polymerase enzymesinitiation complex forms at promoterRNAs are modified after transcription
12 Spliced Gene Transcripts DNA sequence specifying a protein is broken into segments (exons) scattered among longer noncoding segments (introns).Initially, primary RNA transcript is produced for the entire gene.Small nuclear ribonuclearproteins (snRNPs) associate with proteins to form spliceosomes.Excise introns and splice exons to form mature mRNA.
13 RNA SplicingDuring RNA processing, intron sequences are cut out of primary transcript before it is used in polypeptide synthesis.remaining exon sequences are spliced together to form final processed mRNA
16 4. Translation overview a. mRNA attaches to the ribosome b. tRNA's attach to free amino acids in the cytoplasmic "pool" of amino acidsc. tRNA carries its specific amino acid to the ribosome
17 Translation overview (cont.) d. tRNA "delivers" its amino acid based on complementary pairing of a triplet code (anticodon) with the triplet code (codon) of the mRNA.e. Enzyme "hooks" the amino acid to the last one in the chain forming a peptide bond.f. Protein chain continues to grow as each tRNA brings in its amino acid and adds it to the chain. - This is translation!!
18 Gene Expression Translation – in the cytoplasm at ribosome nucleotide sequence of mRNA transcript is translated into amino acid sequence in the polypeptiderRNA recognizes and binds to start sequencemoves three nucleotides at a timedisengages at stop signalGene expression - collective of transcription and translation
19 TranslationBegins when initial portion of mRNA molecule binds to rRNA in a ribosometRNA molecule with complimentary anticodon binds to exposed codon on mRNAsome tRNA molecules recognize more than one codon
20 Translation Start and stop signals Initiation start signal coded by AUG codonstop signal coded by one of three nonsense codons: UAA - UAG - UGAInitiationPolypeptide synthesis begins with the formation of an initiation complex.initiation factors
21 Translation Translocation ribosome moves nucleotides along mRNA molecule
22 Translation Termination Nonsense codons are recognized by release factors that release the newly made polypeptide from the ribosome.
23 Genetic CodeGenetic code consists of a series of information blocks called codons.reading frame (triplet)each codes for one amino acidgenetic code is nearly universalmitochondriachloroplasts
26 The Genetic Code 4 things put together in combinations of 3 = 43= 64 1. A triplet code comprised of three nucleotide bases in a sequence.2. How many triplet codes?20 common amino acids in a protein4 diff. bases on DNA A,T,C, & G| | | |4 diff. bases on RNA U,A,G, & C4 things put together in combinations of 3 = 43= 64Therefore - 64 different DNA triplet codes or RNA codons
27 The 64 triplet codes 60 code for amino acids 4 act as "stop" and "start codesDegenerate Code- more than one triplet code for some amino acids e.g.,GGGGGUGGCGGAAll code for theamino acid glycine
28 Differences Between Prokaryotic and Eukaryotic Gene Expression Most eukaryotic genes possess introns.Individual bacterial mRNA molecules often contain transcripts of several genes.Eukaryotic mRNA molecules must be completely formed and must pass across the nuclear membrane before translation.In prokaryotes, translation begins at the AUG codon preceded by a special nucleotide sequence.
29 Differences Between Prokaryotic and Eukaryotic Gene Expression Eukaryotic mRNA molecules have introns cut out and exons joined together before translation.Eukaryotic ribosomes are larger than prokaryotic ribosomes.