2. Prerequisites (C or better): BSC1010, CHM1046, PCB3063
Cell Biology
PCB 4023
Tu/Th-12:30-1:45 pm
CP 151
Prerequisites (C or better): BSC1010, CHM1046, PCB3063
Capacity
Class is FULL
PCB 4023 Lab ?

3. Course Description
---This is a required course to provide
undergraduate students majoring in
Biology (Marine Biology or others)
with an in depth looks at the
morphology and function
of structures at the cellular level.

4. Course Description
---Topics include
Structure and function of cell membrane systems,
Intracellular transport,
Cellular communication, motility, and division,
HIV retro-virus,
Insulin function and action
Cancer biology.

5. DO something ! Learning Goals
--To gain a solid knowledge base of the structure and function of cells.
--To become familiar
with the appropriate vocabulary.
with the methods used by scientists to study cell biology.
with primary literature in cell biology.
DO something !

6. M. Alejandro Barbieri Office/Lab: AHC-1: 318C / AHC-1:214
Phone: lab
office
(This is the better way to contact me)
Office hours:
-Tu: 2:00 to 4:00 pm
-Th: 3:00 to 5:00 pm
-By appointment ONLY.

7. Course Materials, Text:
Required:
Cell and Molecular Biology, Concepts and Experiments, 7th/ 8th Ed.
by G. Karp
Recommended
Principle of cell biology
By Plopper (2014) ISBN
Biochemistry: A Short Course
By John L. Tymoczko (2009) ISBN
Principles of Cancer Biology
By L. J. Kelinsmith (2006) ISBN
Medical Cell Biology
By S. R. Goodman ( 2008) ISBN
Additional readings will be posted on the website or in the class.
Although these will be announced in class, students are responsible for checking the class web page regularly for announcements and appendices.

8. Textbooks

9. Attendance
Attendance will not be taken but is encouraged. Students are expected to attend class regularly, to arrive on time, and to remain in class until the end of the class period. Class attendance will help you master the course material. Furthermore, exam questions may cover material not found in the text book.
If you miss class, you will still be responsible for any material disseminated or work that is assigned or due in your absence.

10. Grading Policy A 90-100 points B 80-89 points C 70-79 points DF
<60 points

11. Grading Policy Exams: --Four (4) --NO Final exam
--Each exam has a maximum of 20 points …….80 points
--Total points:69
--ONE Class (Special) Assignment
(10 points)
--Four (4) Quizzes
--Each quizz has a maximum of 2.5 points …….10 points
For example
Exam1: 17 pts
Exam2: 20 pts
Exam3: 17 pts
Exam4: 15 pts

12. Grade Curving
=70%
Mean

13. Grade Curving Exam 1 Max: 31 pts Mean= 64.5%
70% represents the mean is 21.7pts
20 pts=Mean of the class is 64.5%
=21.7 pts
20 pts

14. Grade Curving Max: 31 pts 70% represents the mean is 21.7pts Exam 2
30 pts=Mean of the class is 96.7%
Exam 2
Mean=96.7%
Pts=30+0
=21.7 pts
30 pts

15. Grade Curving Exam3 Exam3 Mean= 58.06% Max: 31 pts Pts=18+3.7.=21.7
70% represents the mean is 21.7 pts
18 pts=Mean of the class is 58.06%
Exam4
Max: 31 pts
70% represents the mean is 21.7pts
15 pts=Mean of the class is 48.38%
Exam4
Mean= 48.38%
Pts=15+6.7=21.7

16. Exam 2
Mean= (96.7%)=30 pts
Exam 1
Mean=(64.5%) 20 pts
Student 1= 22 pts + 0= 22 pts
Student 2= 29 pts + 0= 29 pts …
Student 150=24 pts +0=24 pts
Student 1= 20 pts pts = 21.7 pts
Student 2= 28 pts pts = 29.7 pts …
Student 150=25 pts +1.7= 26.7 pts
Exam =3
Mean=(58.06%)= 18 pts
Exam =4
Mean=(48.38%)= 15 pts
Student 1= 15 pts pts= 18.7 pts
Student 2= 20 pt pts= 23.7 pts …
Student 150=22 pts pts= 2.7 pts
Student 1= 12 pts pts= 18.7 pts
Student 2= 20 pt pts= pts …
Student 150=22 pts pts= 28.7 pts

17. Final grade Exam 1 + Exam 2 + Exam 3+ Exam 4 + A + B Quizzes
Special Assignment

18. Exams /Quizzes 1-Four quizzes /exams Quiz 1-Ch1, Ch2, Ch3; 1/31/17
Exam 1- Ch1, Ch2, Ch3; 2/2/17
Quiz 2-Ch4, Ch7, Ch9 – 2/28/17
Exam 2- Ch4, Ch7, Ch9 – 3/2/17
Quiz 3-Ch8, Ch15 – 3/28/17
Exam 3- Ch8, Ch15 – 3/30/17
Quiz 4-Ch12, Ch14, Ch16, Ch17, Ch18 – 4/18/17
Exam 4-Ch12, Ch14, Ch16, Ch17, Ch18 – 4/20/17
Special Assignment -4/6/17
2-Material covered in lecture/ book/ appendices

19. Exams THINK about it Recognize it
3-Exam questions - any combination of multiple choice, true/false and fill in the blank.
4-Exams will be closed book / note / computer / cell phone / etc .
5-You will be asked to show your Panther ID card on exam day so be sure to bring it to class.
6-Two type of questions: Conceptual and Specific
THINK about it Recognize it

20. Special Assignment
Instructions will be sent via on 1/17/17

21. Look for……Teaching… Cell Biology
Look for……Teaching… Cell Biology
General course information including the course syllabus as well as class announcements, chapters and additional required readings.
It is your responsibility to check the course website regularly for updated information and announcements

22. F0 and IN grades
A student who earns a failing grade based on the total number of points and fails to complete at least 60% of the course requirements will receive a grade of F0 (F zero).
A grade of IN (Incomplete) will only be given in extremely rare circumstances where unusual circumstances occur after the Drop date. For a student to receive an IN, the student must have completed at least half the course work and have a passing grade at the time of the incident.
Professional documentation, including an address and telephone number must demonstrate that the student cannot complete the course due to factors beyond their control (severe illness, accident, death in the immediate family, etc.). The student must complete the course within two terms, otherwise the grade will automatically default to an F.

23. Academic Integrity
Collaboration is an important aspect of scientific discovery as well as learning. While it is encouraged that students study together and share data with partners as appropriate, all assignments should be written independently by each student. When appropriate, sources (including the internet and personal communication) should be cited.
Cheating and plagiarism are serious offenses and are NOT acceptable.
If found to have cheated, or have enabled someone else to do so, the student will be subjected to disciplinary action that will include receiving a zero that cannot be dropped for the exam.
A second occurrence will result in the student receiving an F for the course. If you are aware of such academic dishonesty, report it to the professor.

24. Behavioral Misconduct
We (students and instructors) are responsible for maintaining an appropriate, orderly, learning environment in accordance with the mission of the University.
Disruptive behavior will not be tolerated.
All cell phones must be turned off.
During exams all electronic devices must be turned off and put away.
Students in attendance should listen and participate appropriately. Students who fail to adhere to the behavioral expectations outlined by the instructor and presented in the FIU Student Handbook may be subjected to discipline in accordance with the procedures described in the FIU Student Handbook. The student handbook also describes the University's policy on sexual harassment.

25. ADA Accommodation Statement
If the student has a documented disability as described by the Rehabilitation Act of 1973 (PL Section 504) and Americans with Disabilities Act (ADA) and would like to request academic and/or physical accommodations, please contact the Disability Resource Center (tel , TTY/TDD ), as soon as possible. Course requirements will not be waived, but reasonable accommodations be provided as appropriate. It is then the student's responsibility to contact and meet with the instructor as early as possible.

26. Cell Biology
The study of the function, structure and activities of cells--Cellular Level--
Exciting time as disciplines are cooperating to study cells (biologists, chemists, physicists, computer scientists, mathematicians)

27. Soft drinks composition vs. Cell Biology
Source of Energy?

28. SUGARS (high-fructose corn syrup)
Na+/K+
Phosphoric acid
pH~2.1
Caffeine
H20 =
CO2 gas-----CO3H- liquid
Nature’s herbs extract

29. ~ 44 grams
(fructose, glucose and sucrose)
~ 4 to 6 grams/150 lb (68 kg)
(glucose)

30. Intestinal Lumen Blood (?)
SUGARS
Intestinal Lumen Blood (?)

31. Pancreas
Blood beta cells
(sugars) (?)

32. Glucose Regulation of β-pancreatic Cells?
(Blood)
Sugars
Blood

33. SECRETORY-EXOCYTIC
PATHWAY

34. Insulin Receptor-Liver-
Blood

35. SECRETORY-EXOCYTIC
PATHWAY
ENDOCYTIC
PATHWAY

36. Nerves Connect at Synapses

37. Life cycle of a synaptic vesicle
Figure 12-10

38. http://stke. sciencemag. org/content/sigtrans/suppl/2004/12/20/2004

39. SECRETORY-EXOCYTIC
PATHWAY
RECYCLING
ENDOCYTIC
PATHWAY

40. Phagocytosis of Listeria moncytogenes
in macrophages expressing Rab5-GFP

42. Three Americans won the 2013 Nobel Prize in Physiology or Medicine for
discovering the machinery that regulates how cells transport major molecules
in a cargo system that delivers them to the right place at the right time.

44. Cholesterol and Cell Biology
(good, bad, ugly)
186 mg of cholesterol/egg
~35 grams (~10 grams/blood) of cholesterol/150 lb (68 kg)
~1 to 1.5 gram is made by the Liver /day
~25 to 30 % is in your Brain

45. Which one is ….. good, bad and ugly Cholesterol?
Oxidized form
Reduced form
LDL
HDL

49. CHAPTER 1 “Cell”
--The basic structural and functional unit of living organisms
--The smallest structure capable of performing the essential functions characteristic of life
--Others

50. chemistry of living systems
Modern
Cell (and Molecular) Biology
Cytology
the study of
cell structure
Genetics
the study of the
behavior of genes
Biochemistry
the study of the
chemistry of living systems

51. The study of cells
1830’s microscopes had higher magnification and improved resolution (1 micrometer= m)
magnification – the ability to make images of objects larger
resolution – minimum distance that can separate two points that still remain identifiable as separate points when viewed through a microscope

52. The World of the Micrometer
a.k.a. micron, µm
10-6 meter

53. The World of the Nanometer
nm = 10-9 meter
Angstrom (Å) = 0.1 nm

54. The study of cells 1839 – Schwann’s cell theory
All organisms consist of one or more cells
The cell is the basic unit of structure for all organisms
1855 –third component added by Rudolf Virchow
All cells arise only from pre-existing cells
(omnis cellula e cellula)

55. Cell and Molecular Biology* * * * * * *
(*) Appendix