1. BAM: Aerobic Plate Count
1. What does the text say an aerobic plate count (APC) is intended to do?

2. BAM: Aerobic Plate Count
2. What does the text say regarding what two organizations developed procedures for APC?

3. BAM: Aerobic Plate Count
3. If you need more procedural details what source would you use?

4. BAM: Aerobic Plate Count
4. Make a list of the equipment and materials needed for this procedure from Section A.
Underline items you do not have at your work station

5. BAM: Aerobic Plate Count
5.Considering Section A-1 requirements, where in the classroom will you perform these procudures?
6. From section A-1 list three conditions needed for a proper work area

6. BAM: Aerobic Plate Count
7. From section B, What does the word sterile mean? Why is this critical to the procedure?
8. Why should a fallout pour plate be taken during plating?

7. BAM: Aerobic Plate Count
9. After reading section B, determine how many petri dishes will be needed for a
10-6 dilution series?

8. BAM: Aerobic Plate Count
9. After reading section B, determine how many petri dishes will be needed for a
10-6 dilution series?
10. What are other ways to express the term 10-3?

9. BAM: Aerobic Plate Count
11. If we were to use .5 ml of homogenate in our initial dilution, how much diluet should be used? Use an example from section B to support your answer.

10. BAM: Aerobic Plate Count
12. What purpose does agar serve?

11. BAM: Aerobic Plate Count
13. From section B, draw a flow chart of the steps needed to make dilutions down to Ending volumes will be 5ml.

12. BAM: Aerobic Plate Count
14. From section B, draw a flow chart showing how the sample is placed in a petri dish and agar added. Make references to appropriate labs from your lab notebook indicating materials and techniques previously used.