1. A novel site for ubiquitination: the N ‐ terminal residue, and not internal lysines of MyoD, is essential for conjugation and degradation of the protein by Kristin Breitschopf, Eyal Bengal, Tamar Ziv, Arie Admon, and Aaron Ciechanover EMBO J. Volume 17(20):5964-5973 October 15, 1998 ©1998 by European Molecular Biology Organization

2. ATP ‐ and ubiquitin ‐ dependent degradation of wt and mutated MyoD proteins in a cell ‐ free reconstituted system. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

3. In vitro conjugation of wt and lysine ‐ less (0K) MyoD. 35S ‐ labeled proteins translated in reticulocyte lysate in vitro were incubated in a cell ‐ free reconstituted system containing ubiquitin, ubiquitin aldehyde and Fraction II as described in Materials and m... Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

4. In vivo degradation of MyoD ‐ mutated proteins in which the lysine residues were progressively substituted with Arg. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

5. Time course of degradation of wt and lysine ‐ less MyoD in vivo. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

6. Sensitivity of lysine ‐ less MyoD (0K) degradation to different inhibitors. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

7. Detection of ubiquitin–MyoD conjugates in COS ‐ 7 cells. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

8. Selective modification of the α ‐ amino group of the initiator methionine in lysine ‐ less and wt MyoD results in a stable protein that is not conjugated and degraded in vitro. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization

9. In vitro and in vivo stability of wt MyoD with an N ‐ terminal extension. Kristin Breitschopf et al. EMBO J. 1998;17:5964-5973 ©1998 by European Molecular Biology Organization