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Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo by Hanneke Vlaming, Tibor van Welsem, Erik L de Graaf, David Ontoso, AF.

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Presentation on theme: "Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo by Hanneke Vlaming, Tibor van Welsem, Erik L de Graaf, David Ontoso, AF."— Presentation transcript:

1 Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo by Hanneke Vlaming, Tibor van Welsem, Erik L de Graaf, David Ontoso, AF Maarten Altelaar, Pedro A San ‐ Segundo, Albert JR Heck, and Fred van Leeuwen EMBO Rep. Volume ():e201438793 August 20, 2014 ©2014 by European Molecular Biology Organization

2 Location of H2B ubiquitin and its role in H3K79 methylation Nucleosome core particle in which H2BK123, H3K79 and H3K4 are indicated as well as the sites of linear ubiquitin attachment described below. Hanneke Vlaming et al. EMBO Rep. doi:10.15252/embr.201438793 ©2014 by European Molecular Biology Organization

3 Ubiquitin on the H2A N ‐ terminus promotes H3K79 methylation in vivo and in vitro Cartoon of H2A/H2B in the used strains. Hanneke Vlaming et al. EMBO Rep. doi:10.15252/embr.201438793 ©2014 by European Molecular Biology Organization

4 Ubiquitins tethered to the C ‐ terminus of H2A and H2B can modestly promote H3K79 methylation in vivo Cartoon of H2A/H2B in the used strains. Hanneke Vlaming et al. EMBO Rep. doi:10.15252/embr.201438793 ©2014 by European Molecular Biology Organization

5 Tethered ubiquitins can promote H3K4 methylation and DNA damage checkpoint activation Immunoblot analysis of H3K4 methylation in strains expressing various histone ‐ ubiquitin fusions. Hanneke Vlaming et al. EMBO Rep. doi:10.15252/embr.201438793 ©2014 by European Molecular Biology Organization

6 Schematic representation of a crash barrier model for the role of H2Bub in promoting histone methylationUbiquitin, if in the correct orientation, can interact with the nucleosome and coach Dot1 toward H3K79, thereby increasing Dot1′s chances of a productive... Hanneke Vlaming et al. EMBO Rep. doi:10.15252/embr.201438793 ©2014 by European Molecular Biology Organization

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