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ΑKAP is an anchoring protein for a novel CaM kinase II isoform in skeletal muscle by K. ‐ Ulrich Bayer, Klaus Harbers, and Howard Schulman EMBO J. Volume.

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Presentation on theme: "ΑKAP is an anchoring protein for a novel CaM kinase II isoform in skeletal muscle by K. ‐ Ulrich Bayer, Klaus Harbers, and Howard Schulman EMBO J. Volume."— Presentation transcript:

1 αKAP is an anchoring protein for a novel CaM kinase II isoform in skeletal muscle by K. ‐ Ulrich Bayer, Klaus Harbers, and Howard Schulman EMBO J. Volume 17(19):5598-5605 October 1, 1998 ©1998 by European Molecular Biology Organization

2 A model for αKAP as anchoring protein for CaM kinase II. The transcription of the CaM kinase II genes in skeletal muscle (top). K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

3 The CaM kinase II isoforms in a microsomal fraction of skeletal muscle include the novel βM ‐ CaM kinase II. (A) A soluble cytosolic fraction (S) and a particulate microsomal fraction (P) of rat skeletal muscle were compared with recombinant CaM kinase II iso... K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

4 αKAP is particulate and co ‐ localized with CaM kinase II in the SR. (A) A Western blot analysis of a particulate (P) and soluble (S) fraction of skeletal muscle using the NLS antibody is shown and the αKAP band is indicated. αKAP was also probed in fractions... K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

5 αKAP is membrane associated via its N ‐ terminal domain. αKAP was detected by immunoblotting using the NLS antibody. K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

6 αKAP recruits different isoforms of CaM kinase II to the particulate fraction. K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

7 αKAP is associated with CaM kinase II as revealed by an immobilized kinase assay. K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

8 αKAP is binding CaM kinase II at membranes. K. ‐ Ulrich Bayer et al. EMBO J. 1998;17:5598-5605 ©1998 by European Molecular Biology Organization

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