1. July 2002- LJM Introduction to Bioinformatics Lisa Mullan, HGMP-RC

2. July 2002- LJM Bioinformaticians - Develop bioinformatics tools (software) Bioinformatics users - Use bioinformatics tools

3. July 2002- LJM Structure prediction Primer design Sequence alignment Similarity searching Pattern finding Nucleic acid translation Gene prediction

4. July 2002- LJM On the web http://www.ebi.ac.uk http://www.sanger.ac.uk http://www.hgmp.mrc.ac.uk http://www.ncbi.nlm.nih.gov http://www.expasy.ch http://pbil.univ-lyon.fr Or you can download packages of programs onto your own computers

5. July 2002- LJM Most sites use a “sequence search engine” Entrez SRS http://ww.ncbi.nlm.nih.gov/Entrez

6. July 2002- LJM Text (plain) Format MNEGDTHYYNKLPPDSEFGDNHVVILCNM FFHRTEYWWDFCDVNMVCDHGGSAEQQR FASTA Format MNEGDTHYYNKLPPDSEFGDNHVVILCNM FFHRTEYWWDFCDVNMVCDHGGSAEQQR >Sequence description

7. July 2002- LJM Save it as a txt file - Notepad, Teachtext, ASCII format Cut and paste into web based tools - BLAST, PeptideMass Input for programs as part of a larger package - EMBOSS, GCG

8. July 2002- LJM Human Genome Mapping Project – Resource Centre Funded by the Medical Research Council Provide biological and bioinformatics support to the academic community Software development and biological research

9. July 2002- LJM DOSMacOSUNIX Personal ComputersApple MacintoshWorkstations X translates “unix-speak” into DOS and MacOS http://www.hgmp.mrc.ac.uk/Registered/Webapp/vnc/

10. July 2002- LJM cd – change directory cp – copy mv – move or rename rm – delete (remove) pwd – print working directory ls – list (directory contents) mkdir – make a directory rmdir – remove (delete) a directory people/data/ mrna41/ lmullan/ mrna01/ (root) seq.fasta