1. AP Biology 2005-2006 Chapter 43. Immune System lymphocytes attacking cancer cell phagocytic leukocyte lymph system

2. AP Biology 2005-2006 Why an immune system?  Attack from outside  lots of organisms want you for lunch!  animals must defend themselves against unwelcome invaders  viruses protists  bacteria fungi  we are a tasty vitamin-packed meal  cells are packages of macromolecules  no cell wall  traded mobility for susceptibility  Attack from inside  defend against abnormal body cells = cancers

3. AP Biology 2005-2006 Key attributes of immune system  4 attributes that characterize the immune system as a whole  specificity  antigen-antibody specificity  diversity  react to millions of antigens  memory  rapid 2° response  ability to distinguish self vs. non-self  maturation & training process to reduce auto-immune disease

4. AP Biology 2005-2006 Lines of defense  1st line: Barriers  broad, innate, external defense  “barbed wire”  skin & mucus membranes  2nd line: Non-specific patrol  broad, innate, internal defense  “untrained soldiers”  leukocytes (WBCs)  3rd line: Immune system  specific, acquired immunity  “elite trained units”  lymphocytes & antibodies

5. AP Biology 2005-2006 2nd: Internal, broad range patrol leukocytes  innate defense  rapid response  cells & proteins  attack invaders that penetrate body’s outer barriers  leukocytes  phagocytic white blood cells  anti-microbial proteins  inflammatory response  natural killer cells

6. AP Biology 2005-2006 Leukocytes: Phagocytic WBCs  Neutrophils  attracted by chemical signals released by damaged cells  enter infected tissue, engulf & ingest microbes  amoeba-like (fierce!)  lysosomes  ~3 day life span  Macrophages  “big eater”  bigger, long-lived phagocytes

7. AP Biology 2005-2006 Lymph system Production & transport of leukocytes Traps foreign invaders lymph node lymph vessels (intertwined amongst blood vessels)

8. AP Biology 2005-2006 Inflammatory response  Damage to tissue triggers local inflammatory response  histamines & prostaglandins released  capillaries dilate, more permeable  lead to clot formation  increased blood supply  swelling, redness & heat of inflammation & infection  delivers WBC, RBC, platelets, clotting factors

9. AP Biology 2005-2006 Fever  When a local response is not enough  systemic response to infection  activated macrophages release interleukin-1  triggers hypothalamus in brain to readjust body thermostat to raise body temperature  higher temperature helps defense  inhibits bacterial growth  stimulates phagocytosis  speeds up repair of tissues  causes liver & spleen to store iron reducing blood iron levels  bacteria need large amounts of iron to grow

10. AP Biology 2005-2006 3rd: Acquired Immunity  Specific defense  lymphocytes  B lymphocytes (B cells)  T lymphocytes (T cells)  antibodies  immunoglobulins  Responds to…  specific microorganisms  specific toxins  abnormal body cells  antigens

11. AP Biology 2005-2006 “self”“foreign” Antigens  Proteins that serve as cellular name tags  foreign antigens cause response from WBCs  proteins belonging:  viruses, bacteria, protozoa, parasitic worms, fungi, toxins  non-pathogens: pollen & transplanted tissue  B cells & T cells respond to different antigens  B cells recognize intact antigens  invaders in blood & lymph  T cells recognize antigen fragments  invaders which have infected cells

12. AP Biology 2005-2006 Lymphocytes  B cells  mature in bone marrow  humoral response system  “humors” = body fluids  produce antibodies  T cells  mature in thymus  cellular response system  Learn to distinguish “self” from “non-self” antigens during maturation

13. AP Biology 2005-2006 Antibodies  Proteins that bind to a specific antigen  multi-chain proteins produced by B cells  antibodies match molecular shape of antigens  immune system has antibodies to respond to millions of antigens (invaders)  tagging system  “this is foreign!” each B cell has ~100,000 antigen receptors Y Y Y Y Y Y Y Y variable binding region Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y

14. AP Biology 2005-2006 How antibodies work

15. AP Biology 2005-2006 B cell immune response tested by B cells 10 to 17 days for full response invader (foreign antigen) B cells + antibodies Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y recognition Y Y Y Y Y Y Y Y clone 1000s of clone cells Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y plasma cells release antibodies Y Y Y Y Y Y Y Y Y Y Y Y Y memory cells “reserves” Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y YY Y Y captured invaders

16. AP Biology 2005-2006 1° vs 2° response to disease  Memory B cells allow a rapid, amplified response with future exposure to pathogen

17. AP Biology 2005-2006 How can we have so many antibody proteins & so few genes? Light chain Constant region Transcription of gene mRNA Chromosome of undifferentiated B cell B cell C C D J DNA of differentiated B cell Rearrangement of DNA V Translation of mRNA Antibody 40 genes for Variable region Variable region Heavy chain Variable DNA combinations: 1M different B cells 10M different T cells

18. AP Biology 2005-2006 Vaccinations  Active immunity  immune system exposed to harmless version of pathogen  Stimulates immune system to produce antibodies to invader  rapid response if future exposure  Most successful against viral diseases

19. AP Biology 2005-2006 Passive immunity  Maternal immunity  antibodies pass from mother to baby across placenta or in breast milk  critical role of breastfeeding in infant health  mother is creating antibodies against pathogens baby is being exposed to  Injection  injection of antibodies  short-term immunity

20. AP Biology 2005-2006 How are cells tagged with antigens  Glycoproteins on surface of cells have unique “fingerprint”  major histocompatibility proteins (MHC)  human leukocyte antigens (HLA)  MHC proteins constantly export bits of cellular protein to cell surface  “snapshot” of what is going on inside cell MHC proteins displaying self-antigens T cell

21. AP Biology 2005-2006 How do T cells know a cell is infected  Infected cells digest some pathogens & export pieces to MHC proteins on cell surface  antigen presenting cells (APC) T cell MHC proteins displaying foreign antigens invading pathogen T cell antigen receptors

22. AP Biology 2005-2006 T cell response helper T cell infected cell activated macrophage helper T cell stimulate B cells & antibodies Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y YY Y Y killer T cell activate killer T cells or interleukin 1 interleukin 2

23. AP Biology 2005-2006 Attack of the Killer T cells Killer T cell binds to infected cell  Destroys infected body cells  binds to target cell  secretes perforin protein  punctures cell membrane of infected cell Infected cell destroyed Perforin punctures cell membrane Cell membrane Killer T cell Cell membrane Target cell Vesicle

24. AP Biology 2005-2006 Immune response pathogen invasion antigen exposure free antigens in bloodantigens on infected cells humoral responsecellular response B cellsT cells macrophages helper T cells plasma B cells memory B cells memory T cells cytotoxic T cells Y Y Y Y YY Y Y antibodies Y Y Y

25. AP Biology 2005-2006 HIV & AIDS  Human Immunodeficiency Virus  virus infects helper T cells  helper T cells don’t activate rest of immune system: T cells & B cells  also destroy T cells  Acquired ImmunoDeficiency Syndrome  infections by opportunistic diseases  death usually from other infections  pneumonia or cancer

26. AP Biology 2005-2006 Immune system malfunctions  Auto-immune diseases  immune system attacks own molecules & cells  lupus  antibodies against many molecules released by normal breakdown of cells  rheumatoid arthritis  antibodies causing damage to cartilage & bone  diabetes  beta-islet cells of pancreas attacked & destroyed  multiple sclerosis  T cells attack myelin sheath of brain & spinal cord nerves  Allergies  over-reaction to environmental antigens  allergens = proteins on pollen, from dust mites, in animal saliva