1. Microbiology AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 5, part C Microbial Metabolism

2. Feeding methods

4. Chemotrophs Use energy from chemicals. –Chemoheterotroph Energy is used in anabolism. Glucose Pyruvic acid NAD + NADH ETC ADP + PATP

5. Chemotrophs Use energy from chemicals. –Chemoautotroph, Thiobacillus ferroxidans Energy used in the Calvin-Benson cycle to fix CO 2. 2Fe 2+ 2Fe 3+ NAD + NADH ETC ADP + PATP 2 H +

6. Phototrophs Use light energy. Photoautotrophs use light energy in the Calvin- Benson cycle to fix CO 2. Photoheterotrophs use light energy and organic compounds. Chlorophyll Chlorophyll oxidized ETC ADP + PATP

7. Metabolic Diversity Among Organisms Nutritional typeEnergy sourceCarbon sourceExample PhotoautotrophLightCO 2 Oxygenic: Cyanobacteria plants. Anoxygenic: Green, purple bacteria. PhotoheterotrophLightOrganic compoundsGreen, purple nonsulfur bacteria. ChemoautotrophChemicalCO 2 Iron-oxidizing bacteria. ChemoheterotrophChemicalOrganic compoundsFermentative bacteria. Animals, protozoa, fungi, bacteria.

8. Polysaccharide Biosynthesis Metabolic Pathways of Energy Use Figure 5.28

9. Lipid Biosynthesis Metabolic Pathways of Energy Use Figure 5.29

10. Amino Acid and Protein Biosynthesis Metabolic Pathways of Energy Use Figure 5.30a

11. Amino Acid and Protein Biosynthesis Metabolic Pathways of Energy Use Figure 5.30b

12. Purine and Pyrimidine Biosynthesi s Metabolic Pathways of Energy Use Figure 5.31

13. Are metabolic pathways that have both catabolic and anabolic functions. Amphibolic pathways Figure 5.32.1

14. Amphibolic pathways Figure 5.32.2