1. Wards: Natural Selection of Antibiotic Resistance Rich Stein

2. Wards: Lab Aids 36 V 6783 Natural Selection and Antibiotic-Resistant Bacteria Lab Activity 36 V 6783 Natural Selection and Antibiotic-Resistant Bacteria Lab Activity $69.50 $69.50

3. Background Information Antibiotics used to fight infection since 1930s Antibiotics used to fight infection since 1930s Genetic variability Genetic variability Some microbes least resistant or extremely resistance. Some microbes least resistant or extremely resistance. Microbes extremely resistant continue to survive Microbes extremely resistant continue to survive Microbes continue to survive because of resistance or… Microbes continue to survive because of resistance or… Patients not taking ALL of the prescriptions Patients not taking ALL of the prescriptions Surviving microbes live and reproduce Surviving microbes live and reproduce

4. Background Information Leading to classroom discussion about… Leading to classroom discussion about… Many antibiotic resistance diseases: Many antibiotic resistance diseases: Streptococcus pneumonia Streptococcus pneumonia tuberculosis tuberculosis gonorrhea, etc. gonorrhea, etc. Over-prescribing medicine or unnecessary use Over-prescribing medicine or unnecessary use

5. Additions Briefly discuss the role of the immune system as the first line of defense against infectious disease. Briefly discuss the role of the immune system as the first line of defense against infectious disease. Ask students to share their experience with antibiotics. Ask students to share their experience with antibiotics. What illness, what antibiotic, how long, did it work, was all medicine used? What illness, what antibiotic, how long, did it work, was all medicine used?

6. When to apply? Simple evolution activity for the beginning of the unit Simple evolution activity for the beginning of the unit Or ecology unit (population ecology) Or ecology unit (population ecology)

7. What to do… Students work in pairs Students work in pairs One die One die 20 disks 20 disks 13 blue (least resistant bacteria) 13 blue (least resistant bacteria) 6 yellow (resistant bacteria) 6 yellow (resistant bacteria) 1 red (extremely resistant bacteria) 1 red (extremely resistant bacteria) Scenario Scenario Imagine that you are sick with a bacteria infection. Your doctor prescribes an antibiotic to be taken every eight days. Imagine that you are sick with a bacteria infection. Your doctor prescribes an antibiotic to be taken every eight days.

8. What to do… Toss the cube. Toss the cube. You toss What happened What to do 1, 3, 5, 6 You took the antibiotic on time, so bacteria are being killed Remove 5 disks: remove all of the blue first, then yellow, then red 2, 4 You forgot to take the antibiotic Do nothing

9. What to do… Record the number of each bacteria in your body in Table 1. Record the number of each bacteria in your body in Table 1. Bacteria need to reproduce. Add 1 disk of that color to your population. Bacteria need to reproduce. Add 1 disk of that color to your population. If you have resistant yellow, add one yellow If you have resistant yellow, add one yellow If you have extremely resistant red, add one red If you have extremely resistant red, add one red Repeat until you complete Table 1. Repeat until you complete Table 1. Graph “Bacteria Graph” sheet Graph “Bacteria Graph” sheet

10. Changes, modifications, additions Have a group totally skip rolling once, twice, or more. Have a group totally skip rolling once, twice, or more. Classroom discussion about H1N1, news articles about drug resistant tuberculosis. Classroom discussion about H1N1, news articles about drug resistant tuberculosis. http://edition.cnn.com/2000/HEALTH/03/2 4/tb.report/index.html http://edition.cnn.com/2000/HEALTH/03/2 4/tb.report/index.html http://edition.cnn.com/2000/HEALTH/03/2 4/tb.report/index.html http://edition.cnn.com/2000/HEALTH/03/2 4/tb.report/index.html