1. Species identification
How to test meat and animal by-products to confirm they come from the animal stated on the label/documentation?
Important for both food & feed industries

2. Food industry
Undeclared meat in products and meat fraud when more expensive meat is mixed or replaced with cheaper one
Horse-meat scandal in
Europe in 2013
Treacability & testing are crucial to assure consumer safety and trust in food industry!

3. Protein characteristic for a target species
Testing methods
Protein characteristic for a target species

4. ELISA testing solutions for raw and cooked meat speciation
ELISA Technologies manufactures a portfolio of species identification ELISA tests divided into kits for raw, cooked and rendered matrices.
EuroProxima acts worldwide as distributor and provides customer support for these products.

5. ELISA-TEK raw meat
Testing method based on antibodies to species specific serum albumin. Limit of detection: 1% for meat and poultry 1% for milk

6. ELISA-TEK cooked meat
Testing method based on antibodies to species specific muscle-related glycoproteins. Limit of detection: <1% for meat and poultry

7. Feed industry
Due to the outbreak of mad cow disease in the 80s and 90s the EU banned the use of most of the animal by-products in feed for farmed animals
As animal by-products contain a lot of nutrients the EU has been carefully considering the relaxation of the ban

8. Processed animal proteins (PAPs)
Meat and
dairy
production
High temperature
and pressure
By-products
Ruminant processsed animal
protein (ruminant PAP)
High temperature
and pressure
Meat
production
Porcine processsed animal
protein (porcine PAP)
By-products
High temperature
and pressure
Meat and egg
production
Poultry processsed animal
protein (poultry PAP)
By-products

9. Current regulation in the EU
Non ruminant PAPs are allowed in aquaculture feed, ruminant PAPs are not allowed
PAPs intendent for aqua feed and aqua feed itself should not contain ruminant PAPs
In general farmed ruminants and non ruminants cannot be fed with PAPs

10. MELISA-TEK meat species
Testing method based on antibodies to species specific troponin-I (muscle) protein.
Limit of detection: 0.05% in muscle tissue
0.5% in meat and bone meals, feed
(0.1% with high sensitivity extraction)
MELISA-TEK Ruminant kit can be used to monitor the safety of the introduction of processed animal proteins in aqua feed.
No risk of false positive if milk is present in a sample when using MELISA-TEK, in contrast to PCR method

11. End users for the meat speciation ELISAs
Food industry
meat processors
food industry using meat as an ingredient (ready meals, frozen meat products)
food inspectors & control agencies
Feed industry:
PAPs producers
feed producers
Feed inspectors & control agencies

12. Thank you for your attention!
EuroProxima B.V. Beijerinckweg 18, 6827 BN Arnhem The Netherlands Phone: +31 (0) Fax: +31 (0)