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Fig.1 MALDI QqTOF MS spectrum of the tryptic digest of the “52.7 kDa” protein gene_21|conserved hypothetical protein|[Escherichia coli O157:H7 phage Rogue]

Published byMolly Cook Modified over 8 years ago

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Presentation on theme: "Fig.1 MALDI QqTOF MS spectrum of the tryptic digest of the “52.7 kDa” protein gene_21|conserved hypothetical protein|[Escherichia coli O157:H7 phage Rogue]"— Presentation transcript:

1 Fig.1 MALDI QqTOF MS spectrum of the tryptic digest of the “52.7 kDa” protein gene_21|conserved hypothetical protein|[Escherichia coli O157:H7 phage Rogue] 001 MAYTEIAMAV GNSPETLARN VKMMISSGFQ PYGDVQMLSG TLFGMKMAKG TANNVEDFMV 060 TSDTTIQGLV NRVNRYLAQG WRRFGNAHFE DGAYISALAK GDFVSDEGGG SGEAGPQGPV 120 GPAGPAGVAG PQGPQGPQGP AGATGPAGAA GAKGDTGPAG VAGPQGPQGE TGEAGPQGSI 180 GPTGATGPSG PKGDKGDAGP VGPAGLTFRG MYDAATAYVK DDVVTFNSSS WFATTAVTGE 240 NPDISDSWEL LAAQGAPGPQ GATGPTGPTG PAGPAGIQGA QGERGIQGEQ GPSGPQGLQG 300 AAGPVGPQGP AGPQGERGIQ GPVGPQGPIG PKGDKGDAGM SRSFLKVRTS PNTGTNHMIR 360 LPAPLSNISI GIRLDSNIRF SIRAWIDDGT TARTIRGNIE AFNETGVYTN TTVRTSISTS 420 TETGTVLLGD IGEFQGIRPY TLSFYETTTD TMWRATINLF TAGYIVNGNQ ALCIELVRLD 480 A

2 10001500200025003000350040004500500055006000 m/z, amu 0 400 800 1200 1600 2000 Intensity, counts 1298.711 1193.696 1869.028 1145.559 2947.397 1763.990 3988.983 1349.798 2250.108 1466.789 2045.019 3253.529 3675.795 5295.510 5451.638 5825.848 Fig. 2 MALDI QqTOF MS spectrum of the tryptic digest of the “30.5 kDa” protein gene_20 major capsid protein [Escherichia coli O157:H7 phage Rogue] 001 MTIKLDAFEQ EKITNHLRAM GVDKADAAGI WTVKQLTASL NKAYETEYDQ 051 NSVVNLFPVS NEIPGYAKYF EYPVFDGVGI AQIVADYTDD LPLVDALATE 101 RQGKVFRFGN AFLISIDEIK VGQATGQSLS TRKQSLAFEA HDKLLDKLVW 151 SGSTAHGIPS VFDYPNINNV VSGGSWSQPT TAVSDITSLL DIIETSTNGQ 201 HRATHLLLPT TARRIMQNLV PGTSVSYGEF FRQNNSGVTV EFVQYLNDYN 251 GTGTSAAIAY EKDPNNMAIE IPEATNALPA QPKDLHFRVP VTSKATGLIV 301 FRPLTMAVMK GITFA

3 Fig. 3 MALDI QqTOF MS spectrum of the tryptic digest of the “25.9 kDa” protein gene_12, putative major tail protein [Escherichia coli O157:H7 phage Rogue] 001 MAHLSNGTQI FLQGSKSESV AVTAITNAAQ PVMTVADAST FAAGDFIVVE SSSWSKLSEK 061 QLRVVTSTAT TITVEGIDTT DPLQFPAGGT ASIYKVLTWY EMPCVQDVST DGGEQQFVTY 121 QCLADDREQQ IPTYKSAVNT TYTFAHEFTN PIYPVLRNYD ESGALIAIRA YVPKAGEVRL 181 WTGTIAFNET PNVSVNEIET VSVAITVRGR YSFLAA

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