Carbohydrate Identification Lab Results & Conclusion

Results: Which of the solutions reacted to Benedict’s Solution? Monosaccharide-Known Honey Apple Juice Milk (in some classes)

Results: Which of the solutions reacted to Iodine Solution? Polysaccharides Oat Water Rice Water Milk (a little bit)

Results: Which of the solutions did not react at all? Disaccharides Sugar Water

Conclusion: (a) Mono, Di or Polysaccharides? Reaction with Benedict’s  Monosaccharides Present Benedict’s only reacts with Monosaccharides Honey, Apple Juice and Milk contains monosaccharides You saw a orange-yellow-brown color change, resulting from Benedict’s solution!

Conclusion: (b) Structure of Molecules & Benedict‘s Reacted w/Benedict’s  Monosaccharide Monosaccharide = 1 sugar Structure of Honey, Apple Juice & Milk: The carbohydrate was made of individual, single sugar units. Most likely glucose or fructose.

Conclusion: (c) Mono, Di, or Polysaccharides? Reaction with Iodine  Polysaccharides Present Iodine only reacts with Polysaccharides Oat Water, Rice Water & Milk contains polysaccharides You saw a blue-black color change, resulting from Iodine reaction!

Conclusion: (d) Structure of Molecules & Iodine Reacted w/Iodine  Polysaccharides Polysaccharide = Many Sugars Structure of Oat Water, Rice Water & Milk: The carbohydrates were large molecules, made of repeating sugar units. Most likely starchy.

Conclusion: (e) Mono, Di, Polysaccharides? No Reaction  Disaccharide No Color Change Ever  Disaccharide Sugar Water must contain disaccharides

Conclusion: (f) Structure of Molecules & Nada No Reaction  Disaccharides Disaccharide = 2 or Double Sugar Structure of Sugar Water: Made of double sugar units Most likely sucrose or sucralose

Conclusion: (g) Solutions & Long Energy Oat Water & Rice Water: Largest Carbohydrates Most Bonds  Most Energy over Long Period

Conclusion: (h) Solutions & Quick Energy Honey & Apple Juice: Small Carbohydrates Few Bonds  Little Energy over Short Period