IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A.

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IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A

IFNγIL-2TNFaIL-17A CD69 MOPP DMSO CD8+ Supplemental figure 5B SEB isotype control isotype control isotype control B

Supplemental Figure S5. Isotype controls for CD4+ (A) and CD8+ (B) T-cell responses Gates were set on CD3+ lymphocytes, doublets and dead cells were discriminated. CD4+ (A) and CD8+ (B) T-cells were subdivided. CD69 was used as an early activation marker and CD40L - as a marker for T- helper cells which have been recently activated. Subsequent multiparameter flow cytometric analysis was performed according to the expression of IFNγ, IL-2, TNFa and IL-17A. SEB was used as positive control and DMSO as negative control. CD69+ gating strategy was chosen as a representative sample after SEB stimulation. Isotype-matched control antibodies were used to distinguish non-specific (“background”) stainings.