Milsana ® Fungicide Nick Macris Director, Business Development (727) (mobile) (530) (office)

 Source: Extract from giant knotweed Reynoutria sachalinensis (REYSA).  Target pathogens: Milsana ® effectively controls powdery mildew, gray mold, rust, and bacterial blight on ornamental plants and edible crops.  Mode of Action: Induction of plant defense responses. –Reactive oxygen species –Enzymes of the phenolic pathway –Lignification of cell walls –Papilla formation –Phytoalexins  Has EPA crop and non crop registrations as biochemical biopesticide. California - non crop label only. Milsana ® Fungicide Overview

Milsana ® Label Recommendations  Apply at the rate of % in 50 to 100 gallons of water per acre.  Add 0.02% wetting agent such as Nu-Film P.  Use the spray mixture preventatively at the 4-6 leaf plant stage and repeat spraying every 7-14 days depending on disease pressure.

Milsana ® on Ornamentals Powdery Mildew (Oidium spp) Gray Mold (Botrytis cinerea) Rust (Puccinia antirrhini)  Begonias  Lisianthus  Salvias  Crape myrtle  Petunias  Snapdragons  Freesias  Poinsettias  Zinnias  Gerbera  Roses Diseases

Milsana ® on Food Crops  Cucumbers  Melons  Squash  Grapes  Peppers *  Strawberries  Lettuce  Pumpkins  Tomatoes Powdery Mildew (Sphaerotheca spp, Erysiphe cichoracearum ) Gray Mold (Botrytis cinerea) Bacterial blight (Xanthomonas campestris)* Diseases

Summary of Milsana ® Activity Summary of REYSA Extract Activity on Various Edible Crops and Diseases, Crop / PlantDiseasesInduction Activity CucumbersSphaerotheca fuliginea+++ SquashS. fuliginea+++ MelonsS. fuliginea+++ GrapesUncinula necator+++ PeppersXanthomonas spp.++ PumpkinS. fuliginea++ LettuceErysiphe cichoriasearum++ StrawberriesS. macularis++ WheatErysiphe graminis++ BeansBotrytis cinerea Sclerotinia + (-)

Field Data Grape powdery mildew trial, (W.D. Gubler, Univ. of California, Davis, July 2000) Trt#Product, sequence (among 24 combinations tested) Severity %Incidence % 1Untreated62.0 a100 a 2Serenade ® WP + Rally ® WP, 7x4.0 b40.0 b 3Milsana ®, 7x2.2 b28.8 b 4Mankocide ® + Microthiol ® 80 DF, 5x and Sulfur 80 DF, 7x 2.0 b32.5 b 5Sulfur 80 DF 1x followed by Procure ® 50 WS + Sulfur 80 DF,7x 1.3 b21.3 b 6Serenade ® WP, (low) 13x1.2 b33.1 b 7Abound ® 2.08 SC, 7x0.5 b13.8 b

Botrytis disease ratings and yield of “Trust” Tomatoes (Central Mississippi Research Center, 2003) TreatmentDiseaseMarketable yield ratingper plant (lb) (0-100%) Untreated Control 53.3 a 9.6 a Milsana ® 26.7 b 11.4 b Elevate ® 50WG 16.7 b 11.2 b Switch ® 62.5 WG 26.7 b 11.4 b Serenade ® 20.0 b 10.7 a Scala ® 53.3 a 10.7 a Field Data

 Current EPA registration for crop and non crop (ornamentals) uses.  CA non crop label only (MOI will submit crop label by the end of 2007).  More targets will be added (e.g. tomato, turf).  All other crops and diseases are opportunities for IR-4 priorities.  MOI is working on an organic formulation. Regulatory/Toxicology

Potential IR-4 Projects  Test Milsana ® as part of IPM programs in ornamentals, vegetables, fruit, vines (Botrytis, mildews, bacterial diseases. Other diseases ?  Test Milsana ® on turf diseases.  Test efficacy against other pathogens, such as oomycetes (Phytophthora spp. and downy mildew).