Supplemental Figure 1a. SDS-PAGE analysis showing OMPs expressed by V. tubiashii strain 19105. From left to right, Lane 1, cells grown in the presence.

Slides:

Advertisements

Similar presentations

Supplemental Table S1. Up-regulated Genes in OsCc1:AP37 and OsCc1:AP59 Plants in Comp arison to Nontransgenic Controls.

Advertisements

A Look into the Process of Marker Development Matt Robinson.

Supplement Figure 1A. Representative 2D gel image of normal whey with 32 differently expressed protein spots identified by peptide mass fingerprinting.

14.5L/9.5DTime7:00- 9:00 9:00- 11:00 11:00- 15:00 15:00- 17:00 17:00- 19:00 19:00- 21:00 21:00- 23:00 23:00- 5:00 5:00- 7:00 Temperature

Vaccines development of Vibrio vulnificus

Additional file 1 Figure S1 Figure S1. SDS-PAGE analysis of the expression of sbOMT1 and sbOMT3 enzymes in E. coli. The cells were cultured in an LB medium.

Figure S1 pEG + pJG pEG + pJG DUG2 pEGDUG3 + pJG pEGDUG3 + pJGDUG2 pEG202 + pJGDUG WD40 domain pEGDUG3 + pJGDUG2 WD40 domain +ve control -Ve control CM+Leu.

PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1.

Chapter 08 Author: Kelly Elkins © 2013 Elsevier, Inc. All rights reserved.

Group 4 Data Diane Meas The 3 A-Michaels (get it??) 3 amigos… a-michaels….

Supplementary Material The phytohormone ethylene enhances cellulose production, regulates CRP/FNR transcription, and causes differential gene expression.

MdSFBB3-alpha MSHVRESETPEDRVVEILSRLPPKSLMRFKCIHKSWFSLINNLSFVAKHLSNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSMINFSIDSDENNLHYDVEDLN-IP 109 MdSFBB3-beta MSQVHESETPEDKVVEILCRLPPKSLMRFKCIRKSWCTLINRPSFVAKHLNNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSTINLSIDSDEHNLHYDVEDLI-IP.

Supplemental Digital Content 1:

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

PCR Polymerase Chain Reaction

Copyright © 2005 American Medical Association. All rights reserved.

DNA analysis on patients with STS deficiency

FECH gene marker site (Single-plex assay design)

A Highly Sensitive, Multiplex Broad-Spectrum PCR-DNA-Enzyme Immunoassay and Reverse Hybridization Assay for Rapid Detection and Identification of Chlamydia.

Supplemental Figure-Genome alignment of TX16 and Aus0004

Fig. S Fig. S2 Cre-mediated recombination in vivo. G2 mice displaying high levels of GFP were crossed.

© 2013 Elsevier, Inc. All rights reserved.

A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site Shujian Liang, Harold N. Bass,

by Takashi Kasukabe, Junko Okabe-Kado, and Yoshio Honma

Federico Katzen, Jon Beckwith Cell

Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences Marco Musso, Renata Bocciardi, Sara Parodi,

Daniel Chi-Hong Lin, Alan D Grossman Cell

Single Monochrome Real-Time RT-PCR Assay for Identification, Quantification, and Breakpoint Cluster Region Determination of t(9;22) Transcripts Marina.

HKAP1.6 and hKAP1.7, Two Novel Human High Sulfur Keratin-Associated Proteins are Expressed in the Hair Follicle Cortex Yutaka Shimomura, Noriaki Aoki,

Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Yi Wang, Yan Wang, Ruiting Lan, Huaqing Xu, Aijing Ma, Dongxun Li,

Silencing in Yeast rDNA Chromatin

Antigenic Variation in Lyme Disease Borreliae by Promiscuous Recombination of VMP- like Sequence Cassettes Jing-Ren Zhang, John M Hardham, Alan G Barbour,

Genetic characterisation of Helicobacter pylori isolates from an Argentinean adult population based on cag pathogenicity island right-end motifs, lspA-glmM.

(a) (b) FhGALE M U I S W1 W2 E1 E2 E3 M - +

Volume 62, Issue 3, Pages (September 2002)

M. Schwaiger, O. Péter, P. Cassinotti

Properties of H. volcanii tRNA Intron Endonuclease Reveal a Relationship between the Archaeal and Eucaryal tRNA Intron Processing Systems Karen Kleman-Leyer,

Analysis of Proteins with Caseinolytic Activity in a Human Stratum Corneum Extract Revealed a Yet Unidentified Cysteine Protease and Identified the So-Called.

Interaction with PCNA Is Essential for Yeast DNA Polymerase η Function

Volume 17, Issue 1, Pages (January 2010)

Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2

Molecular cloning of pms916 salt hypersensitive T-DNA mutant.

An RNA Sensor for Intracellular Mg2+

Variable flagella expression among clonal toxin A−/B+ Clostridium difficile strains with highly homogeneous flagellin genes H. Pituch, P. Obuch-Woszczatyñski,

A Multi-Exonic BRCA1 Deletion Identified in Multiple Families through Single Nucleotide Polymorphism Haplotype Pair Analysis and Gene Amplification with.

An Important Role for the Multienzyme Aminoacyl-tRNA Synthetase Complex in Mammalian Translation and Cell Growth Sophia V. Kyriacou, Murray P. Deutscher

Rapid Decay of α6 Integrin Caused by a Mis-Sense Mutation in the Propeller Domain Results in Severe Junctional Epidermolysis Bullosa with Pyloric Atresia

A Novel S115G Mutation of CGI-58 in a Turkish Patient with Dorfman–Chanarin Syndrome Zied Ben Selma, Sebnem Yilmaz, Pierre Olivier Schischmanoff, Astrid.

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.

Exon Junction Sequences as Cryptic Splice Sites

B. Bakhshi, M.R. Pourshafie, F. Navabakbar, A. Tavakoli

Cloning of a novel gene in the human kidney homologous to rat munc13s: Its potential role in diabetic nephropathy Yong Song, Menachem Ailenberg, Mel.

The Pro162 Variant is a Loss-of-Function Mutation of the Human Melanocortin 1 Receptor Gene Celia Jiménez-Cervantes, Concepción Olivares, Petra González,

Nucleotide sequence alignment of porA gene sequences from the examined English Neisseria gonorrhoeae porA mutants, compared to the porA sequences of previously.

Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes Karla Cauza, Gabriele Hinterhuber, Ruth Dingelmaier-Hovorka, Karin.

Volume 5, Issue 6, Pages (November 2012)

PCR amplification of the ORF encoding the cytosolic p36 protein of M

Characterisation of eSRS21 protein using an anti-eSRS21 antibody.

Anthony M. Raizis, Martin M. Ferguson, David T. Nicholls, Derek W

Volume 2, Issue 2, Pages (March 2009)

Identification of TSIX, Encoding an RNA Antisense to Human XIST, Reveals Differences from its Murine Counterpart: Implications for X Inactivation Barbara.

Expression of selected genes in the ΔSMcomS strain in response to increasing concentrations of XIP. RT-PCR analysis of gene expression was performed in.

MW (kDa) 2 µg 0.5 µg Supplementary Figure S1. SDS-PAGE under reducing condition analysis (Coomassie brilliant blue staining) of.

Expression of selected intergenic regions in response to increasing concentrations of XIP. qRT-PCR analysis of gene expression was performed with strain.

Molecular Therapy - Methods & Clinical Development

Mutation of the Ca2+ Channel β Subunit Gene Cchb4 Is Associated with Ataxia and Seizures in the Lethargic (lh) Mouse Daniel L Burgess, Julie M Jones,

Volume 1, Issue 1, Pages (January 2008)

Exon Skipping in IVD RNA Processing in Isovaleric Acidemia Caused by Point Mutations in the Coding Region of the IVD Gene Jerry Vockley, Peter K. Rogan,

Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International

Presentation transcript:

Supplemental Figure 1a. SDS-PAGE analysis showing OMPs expressed by V. tubiashii strain From left to right, Lane 1, cells grown in the presence of 3% NaCl compared to those grown with 1% NaCl in Lane 2; Lane 3 cells grown in the presence of 0.3% bile salts compared to those grown without bile salts Lane 4; Lane 5 cells grown in the presence of 2% maltose compared to those grown without maltose Lane 6; Lane 7 represents the molecular markers, sizes are reported in kDa. Note the sequenced and uniquely expressed OMPs at each environmental condition are identified with an arrow. 50 kDa 70 kDa 45 kDa 40 kDa 90 kDa 25 kDa 20 kDa 15 kDa 65 kDa 35 kDa Bile salts 0.3%0% NaCl 3% 1% Maltose 2% 0% MW 1a.

Temperature 37° 30° pH MW 50 kDa 70 kDa 45 kDa 40 kDa 90 kDa 25 kDa 20 kDa 15 kDa 65 kDa 35 kDa Supplemental Figure 1b. SDS-PAGE analysis showing OMPs expressed by V. tubiashii strain From left to right, Lane 1-2, cells grown at different temperatures; and Lane 3-5, cells grown at various pH conditions; Lane 6 represents the molecular markers, sizes are reported in kDa. Note the sequenced and uniquely expressed OMPs at each environmental condition are identified with an arrow. 1b.

Supplemental Figure 2a. SDS-PAGE analysis showing OMPs expressed by V. tubiashii strain From left to right, Lane 1, cells grown in the presence of 3% NaCl compared to those grown with 1% NaCl Lane 2; Lane 3, cells grown in the presence of 0.3% bile salts compared to those grown without bile salts Lane 4; Lane 5, cells grown in the presence of 2% maltose compared to those grown without maltose, Lane 6; Lane 7 represents the molecular markers, sizes are reported in kDa. Note the sequenced and uniquely expressed OMPs at each environmental condition are identified with an arrow. 50 kDa 70 kDa 45 kDa 40 kDa 90 kDa 25 kDa 20 kDa 15 kDa 65 kDa 35 kDa NaCl 3% 1% Bile salts 0.3% 0% Maltose 2% 0% MW 2a.

Temperature 37° 30°43° pH MW 50 kDa 70 kDa 45 kDa 40 kDa 90 kDa 25 kDa 20 kDa 15 kDa 65 kDa 35 kDa Supplemental Figure 2b. SDS-PAGE analysis showing OMPs expressed by V. tubiashii strain From left to right, Lane 1-3, cells grown at various temperatures; and Lane 4-6, cells grown at various pH conditions; Lane 7 represents the molecular markers, sizes are reported in kDa. Note the sequenced and uniquely expressed OMPs at each environmental condition are identified with an arrow. 2b.

a. Vv4965-T1CTCTAGAAGTTGGTGGCCGTGCTGAAGCTCGCCTATCTCTGAAAGATGGTAAGGCACAAG Vc395 CTCTAGAAGTTGGTGGCCGTGCTGAAGCTCGCCTATCTCTGAAAGATGGTAAGGCACAAG Vt109 CTCTAGAGATCGGCGGTCGTGCTGAAGCTCGTATGTCTCTAAAAGATGGTAAAGCTGAAG Vt105 CTATCGAAATGGGTGGTCGCGCGGAAGCACGTCTAGCTCTTAAAGATGGNAAGGCAGAAN ** * ** * ** ** ** ** ***** ** * **** ******** ** ** ** Vv4965-T1 ACAACTCTCGCGTACGTCTAAACTTCTTGGGTAAAGCAGAAATCAATGACAGCCTATACG Vc0395 ACAACTCTCGCGTACGTCTAAACTTCTTGGGTAAAGCAGAAATCAATGACAGCCTATACG Vt109 ACAAAACACGTGTACGTCTAAACTTCCTAGGTAAGACTCAAATCACTGATAGCCTATATG Vt105 ACAAATCTCGCGTACGTCTAAACTTCCTAGGTACAGCTCAAATCACTGACAGCCTATACG **** * ** *************** * **** * ****** *** ******** * Vv4965-T1 GTGTTGGTTTCTACGAAGGCGAGTTTACTACTAATGATCA AGGTAAAA Vc395 GTGTTGGTTTCTACGAAGGCGAGTTTACTACTAATGATCA AGGTAAAA Vt109 GTCTAGGTTTCTACGAAGCTGAGTACACTACAGCAGACAACACTGTAACTGTAGACGGCA Vt105 GTGTGGGTTTCTACGAAGGTGAGTTCACTACTAATGACAATGGCGGCGCTACAGATGGCA ** * ************* **** ***** ** * ** * Vv4965-T1 ACG-CGTCTAACA---ACAGCCTANACAACCGTTATACCTACGCTGGTATCGGTGGCACT Vc395 ACG-CGTCTAACA---ACAGCCTAGACAACCGTTATACCTACGCTGGTATCGGTGGCACT Vt109 AAA-CAACTGACGGTGATAACATTGAGCACCGTTACACTTACGCTGGTATTGGTGGTGAC Vt105 ACAACAGCTCATC----TGATCTAACTAACCGTTACCTATACGGTGGTATCGGCGGAGCT * * ** * * ******* **** ****** ** ** Vv4965-T1 TACGGTGAAGTGACTTACGGTAAAAACGATGGCGCATTGGGCGTAATCACTGACTTCACC Vc395 TACGGTGAAGTGACTTACGGTAAAAACGATGGCGCATTGGGCGTAATCACTGACTTCACC Vt109 TTTGGTGAAGTAACTTACGGTAAAAACGATGGTGCACTANGCGTTATCACTGACTTTACT Vt105 TTCGGTGAAGTAACTTACGGTAAGAACGACGGTGCGCTANGTGTTATCACTGATTTCACT * ******** *********** ***** ** ** * * ** ******** ** ** Supplemental Figure 3. Clustal X alignment of nucleotide sequence of ompU gene from V. tubiashii strains and 19109, V. vulnificus 4965-T1 and V. cholerae 395, respectively. ompU gene sequences were obtained in this study. Indicated by underlying are the PCR primer sequences used in the study. a. Forward sequence, b. Reverse sequence. The symbol “*” represents conserved nucleotides among the sequences.

Vv4965-T1 NCNNTGGACTGACC-CAGTTCGTC-TTCTGATGCTACTTTACCAA CTTTG Vc395 GCNNNGNANTTANG-CAATACNTNGTTCTGATGCTACTTTACCAAA CTTTG Vt109 GCNNNGAACTGAANATAGCTCGTC-TTCTGCACCAGCTTTACCTACAGTACCAGTTGCTG Vt105 NCCNTNGACNNAAA-AAGCTCGTC-TTCTGCATCTGCTTTAGAGATG----TAAGATTTG * * * * * * ***** * ***** * ** Vv4965-T1 TCTGA--ATCT------AGCAGATTGAACTGGTAAGAGATGTAAGAGCGNNAGTTTGGCT Vc395 GCTGA--ATCT------AGCAGATTGAACTGGTAAGAGATGTAAGAGCGNAAGTTTGGCT Vt109 TCTTATCATCTTTACTAAGTAGGTTGAAGTTGTAAGAGATGTAAGTACGGAAGTTTGGCT Vt105 TCGTC--GTCT------AGCAGGTTAAAGTTGTACGATACGTAGNCACGGAAGTTAGGCT * *** ** ** ** ** * *** ** * *** ** **** **** Vv4965-T1 TGAAGTAGTAAGTTGCGTCGATAGCAAAATTATCTGCTGAAGT T-TCTTTCG Vc395 TGAAGTAGTAAGTTGCGTCGATAGCAAAATTATCTGCTGAAGT TCTCTTTCG Vt109 TGAAGTAGTATGTTGCATCGATCGCTACATTGTCAGCTGCATCATACGTAGTACCTGAAG Vt105 TAAAGTAGTATGTTGCGTCGATAGCGATGTTATCTGCAGCATCTTCTTTAGTTGAACCTG * ******** ***** ***** ** * ** ** ** * * * * Vv4965-T1 CTGTTTCTGCA---TTGTTGTATGTCGCAGTAAACGCAGCTTGACCTAGTTTGTAACCAG Vc395 CTGTTTCTGCA---TTGTTGTATGTCGCAGTAAACGCAGCTTGACCTAGTTTGTANCCAG Vt109 TTTTCTCTTCTAGCTTATTGTATGTTGTAGTGAATGCTACTTTTTCTAGAGTGTAAGCTG Vt105 TTTTGTTTTCTAGGTAGTTGTAAGACGTTGTGAATACAGTCTGGCCCATAGTGTAAGCTG * * * * * ***** * * ** ** * * * * ***** * * Vv4965-T1 CAGCTAGCTCGTAGCCAGTGTAGTCAACGT-----CTTTCGC---TAGCTC AC Vc395 CANCTANCTCGTAGCCAGTGTAGTCAACGT-----CTTNCGC---TAGCTC AC Vt109 CTGCGAATTCGTAACCAGTTTTGTCTGTTTT---GCTTGAGC---CGTCTTTGTCTTTAC Vt105 CTGCTAGTTCGTAACCTGTCTTATCAGTTGTTGAGCTAGAACCTTTCACTTGGTCTTCGC * * * ***** ** ** * ** ** * ** * b. Supplemental Figure 3. Clustal X alignment of nucleotide sequence of ompU gene from V. tubiashii strains and 19109, V. vulnificus 4965-T1 and V. cholerae 395, respectively. ompU gene sequences were obtained in this study. Indicated by underlying are the PCR primer sequences used in the study. a. Forward sequence, b. Reverse sequence. The symbol “*” represents conserved nucleotides among the sequences.

Vt109 NCNNNNTCCNNNNCTTACCCAGCCTGACTCAAGCCATCTCAACCCTACGTAAAATGCTGA Vt NCNNNTTCAAAACCTGACTCAGGCCATCTCATCCCTACGTAAAATGCTGA Vf TGGTGGAATGCACACCGGCGGACTCGGCCCCAAAGAAGTCATCGC * ** * ** * *** *** * ** * Vt109 AAGACTCGACCAAGTCGCC--TCAGTTTGTGAAA-ACGGTTCCAAAACGTGGTTATCAGT Vt105 AAGACTCGACTAAATCCCCCATCAATCCGTGGAAGACGGTTCCAAAACGTGGTTATCAGT Vf33809 TACCGGCGGTCAAAAC * ** ** Vt109 TGATCGCGACCGTCGAAAGCGTTCAGCTCGATGCAAGTAAAGATCCTGATGCCATCGAGC Vt105 TGATCGCGACCGTCGAAACCCTGGAGCTCGATGCAAGTAAAGATCCTGATGCCATCGAGC Vf Vt109 AGTCAGATGAGTCTTACTCAAGTGCCGTATCCTCTTCAGAACTTGTCAGTCAAGCTGCTG Vt105 AATCATATGAGTCTTACTCAAGTGCCGTATCCTCTTCACAACTTGTCAGTCAAGCTGCTG Vf Vt109 TTTCTGAAGCAGTTATTACTCAGACGTCGCCTTCTGTAGACTCGACACCTGCGGCACCAA Vt105 TTTCTGAATCAGTTATTACTCAGACGTCGCCTTCTGTAGACTCGACACCTGCAGCACCAA Vf Vt109 AACCACGAATGGATCTGTTCACTAAATTGATTCTGTTGGTTGCCGTCATACTGCCAT--- Vt105 AACCACGAATGGATCTGTTCACTAAATTGATTCTGTTGGTTGCCGTCATACTGCCATCNN Vf a. Supplemental Figure 4. Clustal X alignment of nucleotide sequence of toxR gene from V. tubiashii strains and compared to that of V. cholerae 395 and V. fluvialis strain ATCC toxR gene sequences were obtained in this study and gene sequence for V. fluvialis strain ATCC was obtained from the NCBI accession number AF a. Forward sequence, b. Reverse sequence. The symbol “*” represents conserved nucleotides among the sequences.

Vt109 TGAACAGATCCATTCGTGGTTTTGGTGCCGCAGGTGTCGAGT-CTACAGAAGGCGACGTC Vt105 TAAAGGATAACCGTAAGGATGTCGGAGTTCCAATTATTACGCACTCCAGCAGTCGATGTG Vf33809 TGGACGACTCCAGCCTGACTCAAGCCATCTCAACC CTACGTAAAATGCTGAA * * * * * ** ** * * * * Vt109 TGAGTAATAACTGCTTCAGAAACA GCAGCTTG-ACTGACAAGTTCTGAAGAGG Vt105 TGAGCCAAGAGGACTCCATGAACACTTAGNCGCGGTTTCTGCGGACCGATTCCGTGAAGG Vf33809 AGACT--CGACCAAGTC---GCCT CAGTTTGTGAAAAC-GGTTCCAAAACGT ** * * * * ** * *** * Vt109 AT ACGGTACTTGAGTAAGACTCATCTGACTGCTCGATG--GCATCAGG Vt105 ATGGATAGGGCCGGACGATAATTGTGCAAAATTAACTTGACTGCTCGATTATGCATCAGG Vf33809 GGTTATCAGTT--GATCGCGACCGTCGAAAGCGTTCAGCTCGATGCAAGTAAAGATCCTG * * ** * * * ** * Vt109 ATCT-TTACTT--GCATCGAGC-TGAACGCTT-TCGACGGTCGCGATC--AACTGATAAC Vt105 ATCTATTACCTATGCATCGAGCCTGAACGCTTATTAACGCTCGCTACCCNGAGTGATAAC Vf33809 ATGC--CATCGA-GCAGTCAGA-TGAGTCTTACTCAAGTGCCGTATCC--TCTTCAGAAC ** * *** ** *** * * * ** * * * *** Vt109 CAC---GTTTTGGAACCG--TTT-----TCACAAACTGAGGCGACTTGGTCGAGT----- Vt105 CACAGTGGTTGGTAACCGGATTTGNCGATCATAAATGGGTGCGACTTGGTGGTCTAGCTC Vf33809 TTGTCAGTCAAGCTGCTG---TT-----TCTGAAGCAGTTATTACTCAGACGTCG----- * * * * ** ** ** * *** * * Vt109 -CTTTCAGCATTTTACGTA------GGGTTGAG-ATGGCTTGAGTCAGGCTGGA--GTCG Vt105 GCTCTCAAACTTTTACAAACGTTAGGGGTTGAACATGGCTTGACTCAGGCTGCAACGTCT Vf CCTTCTGTAGACTCGACACCTGCGGCACCAAA---ACCACGAATGGATCTGTT----CA * ** * * * ** *** * Vt109 TCCACCT-CAAACCCCTGAG---AATACGTCTCCCNCNCTCTTNCAATCTCACAGTTCCA Vt105 TCCACCTTCAAACCCCTGAACCAAATACCTGTACTNCNCTCTTNCGTTTCCATAGTCACA Vf33809 CTAAATT--GATTCTGTTGGT--TGCCGTCATACTGCCTATTTGTGTCATTTTGTTTACG * * * * * * ** * * b. Supplemental Figure 4. Clustal X alignment of nucleotide sequence of toxR gene from V. tubiashii strains and compared to that of V. cholerae 395 and V. fluvialis strain ATCC toxR gene sequences were obtained in this study and gene sequence for V. fluvialis strain ATCC was obtained from the NCBI accession number AF a. Forward sequence, b. Reverse sequence. The symbol “*” represents conserved nucleotides among the sequences.