Homework 3 1.Plot the gene expression profile of the assigned 4 genes from NCI60. 2.Intersect the same genes from SOURCE and protein-protein interactions.

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Presentation transcript:

Homework 3 1.Plot the gene expression profile of the assigned 4 genes from NCI60. 2.Intersect the same genes from SOURCE and protein-protein interactions.

click

Click color bar

Similar expression genes

Download raw data

1. Download raw data 2. The data will be a.zip file. 3. There will be 68 separate files in there and will be difficult to retrieve your genes 68 times.

To obtain a single “cleaned” file, you need to use the following steps. Cleaned file=normalized and retrieved file.

Select

Find the NCI60 article

Select

1. Select all 3. Select 2. Select

1. Cancel the selection

Wait for results. It may take more than 4 hrs. Here are different steps for data retrieval.

Select

1. Select all data 2. Open a new excel file and paste 3. See next page

1.Paste to Excel 2.Find genes (ie. stk6) from column B Name in excel 用 ‘ 編輯 ’ 選項中 ‘ 尋找 ’ 功能 3.Plot the figure

Please plot your assigned genes by Excel

TACC3 Hec Nuf2  CENP-A  TPX2   HURP LymphomaCarcinoma classification NCI60Normal Tissue Atlas HeLa Cell cycle database substrate