AUC - Past & Present Stephen Harding

Thé Svedberg

Svedberg oil-turbine ultracentrifuge

“Evolution” of the AUC Numbers of papers in the Biochemical Journal, per annum

1920’s: “Thé: I see a new dawn! ” Svedberg & Nichols: oil turbine u/c Optical system Rinde: sector shape cell Hemoglobin - Svedberg & Fåhraeus

1926: 1 st AUC paper on sedimentation equilibrium

1930’s: “Proteins are molecules” Philpott/Svensson Schlieren optics Proteins as molecules Concept of subunits Perrin: Frictional ratio calculations and shapes of molecules: TMV

1930’s: “Proteins are molecules” Philpott/Svensson Schlieren optics Proteins as molecules Concept of subunits Perrin: Frictional ratio calculations and shapes of molecules: TMV

1930’s: “Proteins are molecules” Philpott/Svensson Schlieren optics Proteins as molecules Concept of subunits Perrin: Frictional ratio calculations and shapes of molecules: TMV

1948: Discovery of electricity is taken into account Spinco Model E (Pickels) Beckman Model E (Pickels)

1948: Analysis of Mixtures A.G. “Sandy” Ogston

“Evolution” of the AUC Numbers of papers in the Biochemical Journal

YphantisSchachman Williams van Holde

“Evolution” of the AUC Numbers of papers in the Biochemical Journal

1980’s: “ Take our advice and switch to more useful techniques like NMR” “Advice” from the Biochemical Journal Garcia de la Torre: bead models National Centres in the USA and UK 1989 – news of another “new dawn”

April 1991