Manipulating and Cloning DNA. Being Healthy Type 1 diabetes Type 2 diabetes How can you help these individuals?

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Presentation transcript:

Manipulating and Cloning DNA

Being Healthy Type 1 diabetes Type 2 diabetes How can you help these individuals?

Insulin Banting and Best – Pigs and cows Helen Free Allergic reaction to insulin from pigs and cows Genetic engineering – Inserted into bacteria – Produced insulin from humans

Why Bacteria? Reproduce quickly Plasmids Form recombinant DNA – Restriction enzymes – Sticky ends vs. blunt ends – Restriction fragments Sticky or Blunt ends?

How Do You Know Where to Cut? Restriction enzymes recognize recognition sites – Sticky ends & blunt ends Restriction map

We Are Family Vector + foreign material = recombinant DNA – Plasmids Competent/host cell takes up recombinant DNA – Bacteria Restriction enzymes cut DNA ligase reforms backbone

Recombinant DNA

Hybridization

Homework Page 375 # 2, 3, 5, 8

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