Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage Stage Clips Coarse Focus Fine Focus Base Diaphragm Light/Mirror

3 1) Eye Piece –  the lens that you look through  the tube that supports the eyepiece 2) Body Tube –

4 Revolving 3) Revolving Nosepiece- the rotating device that holds and changes the objectives (lenses).

5  magnification lens with the lowest power 4) Low Power Objective lens – 6) Stage Clips – clips that keep the slide or specimen from moving on the stage  magnification lens with the highest power 5) High Power Objective lens –

6 7) Stage – holds the specimen

7 controls the amount of light entering through the aperature to the objective from the bottom 8) Diaphragm-

8 supports the microscope 10) Light/Mirror 9) Base- reflects (bends) the light upward onto the slide Base Light/Mirror

9 11) Fine Focus Adjustment – 12) Coarse Focus Adjustment- 13) Arm – moves the body tube in small increments moves the body tube in large increments attaches the eyepiece and the body tube to the base Arm Coarse Focus Fine Focus

1) Place the Slide on the Microscope 2) Use Stage Clips 3) Click Nosepiece to the scanning objective 4) Look into the Eyepiece and adjust the slide until object is centered 5) Use the Coarse Adjustment Knob to bring into focus 6) Use the Fine Focus Knob to bring the slide into focus 7) Click nosepiece to low power objective 8) Use Fine Focus knob to bring slide into focus

1) Make sure the object is centered and focused at scanning and low power 2) Click the nosepiece to the high power objective (largest) lens 3) Use the Fine Focus Knob to bring the slide into focus **Do NOT use the Coarse Focusing Knob** **Never remove a slide while under high power**

 Your microscope has 3 magnifications: ◦ Scanning= 4 ◦ Low = 10 ◦ High= 40  The eyepiece has a magnification of 10  The total magnification = the eyepiece times the objective lens ◦ Scanning= 40 ◦ Low=100 ◦ High= 400

 Q: Image is too dark!  A: Adjust the diaphragm, make sure your light is on  Q: There's a spot in my viewing field, even when I move the slide the spot stays in the same place!  A: Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens  Q: I can't see anything under high power!  A: Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power  Q: Only half of my viewing field is lit, it looks like there's a half-moon in there!  A: You probably don't have your objective fully clicked into place