Strawberry DNA Extraction. DNA: Basics Deoxyribonucleic acid Genetic material ◦ Chain of molecules linked together ◦ DNA contains instructions for traits.

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Presentation transcript:

Strawberry DNA Extraction

DNA: Basics Deoxyribonucleic acid Genetic material ◦ Chain of molecules linked together ◦ DNA contains instructions for traits Wrapped tightly around “histones” Forms chromosomes Housed in nucleus

DNA: Basics

DNA: Structure Double-helix (twisted ladder) Sugar and phosphate backbone Nitrogen bases Subunit: “nucleotide”

DNA: Replication Special pairing between nitrogen bases New strand is copy of old strand

DNA Extraction: Step 1 Break the cells open (using force)

DNA Extraction: Step 2 Dissolve the cell membrane and nuclear envelope (using detergent/soap) Current Protocols in Protein Science Published by John Wiley & Sons, Inc.

DNA Extraction: Step 3 Remove proteins that are bound to DNA (using salt to dissolve them)

DNA Extraction: Step 4 Precipitate DNA (with ethanol)

DNA Extraction: Why? Species identification VS.

DNA Extraction: Flow chart