DNA EXTRACTION JAN 24, 2013 Lab # 33

I.Background Information *Strawberries are octoploid, which means they have eight sets of chromosomes. The procedure for extracting DNA from a strawberry is simple, and the results are usually obvious, it is easy to see the white strands of DNA within the pink solution of strawberry juice. *Strawberries are octoploid, which means they have eight sets of chromosomes. The procedure for extracting DNA from a strawberry is simple, and the results are usually obvious, it is easy to see the white strands of DNA within the pink solution of strawberry juice. This process is DNA fingerprinting, It is what Forensic Scientists do. This process is DNA fingerprinting, It is what Forensic Scientists do.

I.Question  What does DNA Look like?

II.Hypothesis If… Then… because… If… Then… because…

III.Materials See Student Manual Page 19 or look at materials you use See Student Manual Page 19 or look at materials you use Make sure to write down all materials

IV.DNA Extraction Techniques Procedure : Extraction from Strawberry DNA Extraction Buffer (can be prepared in advance) DNA Extraction Buffer (can be prepared in advance) 900 ml of deonized water, 50 ml of clear dishwashing detergent, 2 teaspoons of salt 900 ml of deonized water, 50 ml of clear dishwashing detergent, 2 teaspoons of salt 1. Add a strawberry (or half a strawberry) to a ziploc storage back and use your hands to smash (Maceration) the strawberry, taking care to not break the bag. 1. Add a strawberry (or half a strawberry) to a ziploc storage back and use your hands to smash (Maceration) the strawberry, taking care to not break the bag. 2. Add 10 ml of the DNA extraction buffer (Lysis Solution). 2. Add 10 ml of the DNA extraction buffer (Lysis Solution). 3. Smush (Macerate) the strawberry and buffer for one minute. 3. Smush (Macerate) the strawberry and buffer for one minute. 4. Use a coffee filter, cup, and rubber band to filter the strawberry juice into a single cup and then pour into a single test tube. 4. Use a coffee filter, cup, and rubber band to filter the strawberry juice into a single cup and then pour into a single test tube. 5. Slowly pour cold ethanol or 91% isopropyl alcohol (7-10mls) over the top of the strawberry mixture. You want a single layer of clear ethanol on top of the strawberry mixture. COLD ethanol will work best- It condenses the DNA. 5. Slowly pour cold ethanol or 91% isopropyl alcohol (7-10mls) over the top of the strawberry mixture. You want a single layer of clear ethanol on top of the strawberry mixture. COLD ethanol will work best- It condenses the DNA. 6. White strands will form in the ethanol layer, use a stirring rod to spool the strands. 6. White strands will form in the ethanol layer, use a stirring rod to spool the strands.

V.Data This will be a drawing of the DNA when you have completed the lab This will be a drawing of the DNA when you have completed the lab _____________________ 0 X Actual Size STRAWBERRY DNA

V.Data Analysis 1. Why do you think we added the Lysis solution? 1. Why do you think we added the Lysis solution? 2 Why does the isopropyl alcohol need to be cold? 2 Why does the isopropyl alcohol need to be cold? 3. Where do you find DNA? 3. Where do you find DNA? 4. What does DNA look like? 4. What does DNA look like?

VI.Conclusion Next time I would…. Next time I would…. Today I learned…. Today I learned…. My hypothesis was …. My hypothesis was …. Vocabulary Vocabulary 1- Lysis 2-Masuration 3- Spooling 1- Lysis 2-Masuration 3- Spooling