1. Strawberry Fields Forever
PRESENTED BY
LORI NEWMARK, NBCT JOHNNIE KEEL, NBCT
Oakridge Elementary, Moore Truman Elementary, Norman

2. Strawberry Lapbook

3. The Art of Growing Things

6. EXTRACTION 1 strawberry Clear laundry detergent (5 mL)
Water (45 mL)2 tsp of salt
Ice cold rubbing alcohol (25 ml or 5 tsp)
1 Ziploc® bag
1 coffee filter Funnel
Tweezer (or popsicle stick)
Collection cup

7. Safety First Put strawberry in bag. Remove air and seal.
Gently squeeze breaking up all the big chunks.

8. Add 2 teaspoons (5 mil soap)
Noble Foundation suggests clear soap (Woolite)
3 Tablespoons Water
(45 mil water)
Add 2 teaspoons salt. Stir GENTLY to mix the solution.
Lysis Buffer
The soap breaks the cells open. In order to get to the DNA, we have to break the cells open.
Salt helps the DNA precipitate out of the solution at the end of the experiment. Mix gently, no bubbles.
Precipitate: the process of a solid forming out of a solution

9. Add Lysis Buffer to the strawberry
Close bag and gently knead the bag. The cells are breaking open to separate the DNA.
Add Lysis Buffer to the strawberry
You will still see chunks of the berry. We do not need those so we will filter them out of the solution.

10. Open up the outside flap and form a funnel
Open up the outside flap and form a funnel. Place inside the plastic funnel.
Flatten out a coffee filter. Fold it in half, crease the edge and fold in half again forming four quarters.

11. FILTRATE: material that has passed through a filter
Pour the solution into the filter and collect it in the cup. We are now collecting what is called a Filtrate.
It will take minute for all to drain. Can help by squeezing gently but avoid breaking the paper filter or squeezing chunks into the cup.
FILTRATE: material that has passed through a filter

12. MUST BE COLD
Pour 5 teaspoons (25 mil) alcohol into cup

13. Strawberries are octoploids (8 copies of DNA).
Solution becomes cloudy on top. This is the DNA separating from the solution.
Strawberries are octoploids
(8 copies of DNA).

14. STRAWBERRY SALSA
1 pint strawberries, hulled and finely diced
1 jalapeno, stem and seeds removed, finely diced
Half of small red onion, peeled & finely diced (about 1/2 cup)
2/3 cup finely chopped fresh cilantro, loosely-packed
Juice of lime, about 2 Tablespoons
Pinch of salt and black pepper
Toss all ingredients together until combined. Season with extra salt and pepper if desired. If you like a sweeter salsa, stir in a few teaspoons of warmed honey.
Serve with tortilla chips.

15. Bibliography and Resources
Oklahoma Ag in the Classroom Lessons
The Art of Growing Things
DNA Blueprint for Life
Garden Grid
Garden in the Mailbox
Oklahoma’s Berry Best
Noble Research Institute

16. Thank you for coming.
Have a great year!