New Sampling/Sensor Initiative (NeSSI™) Analytical Applications and Web Tools Dave Veltkamp, CPAC

Overview of Talk Discuss how NeSSI™ can facilitate laboratory applications and how current development fits lab needs Examples of NeSSI™ as a platform for micro analytical devices and analyzers Mention some web-based resources at CPAC

What does NeSSI™ Provide Simple “Lego-like” assembly Easy to re-configure No special tools or skills required Standardized flow components “Mix-and-match” compatibility between vendors Growing list of components Standardized electrical and communication (Gen II) “Plug-and-play” integration of multiple devices Simplified interface for programmatic I/O and control Advanced analytics (Gen III) Micro-analyzers Integrated analysis or “smart” systems

NeSSI™: Enabler for MicroAnalytical (the “rail” concept) Standard Mechanical Interface “Rail” Standard Electrical (Digital) Interface “Rail” Anyone’s Sensor Anyone’s Actuator SAM* Standard “hockey-puck PC” P V *Sensor/Actuator Manager Standard “connectivity ”

Where Does NeSSI™ Fit in the Lab Instrument/Sensor Interfaces Design standards make development simpler Reduced toolset to be mastered Reduced toolset to be mastered Reduced sample variability to account for Reduced sample variability to account for Calibration/validation built-in Consistent physical environment for measurement Consistent physical environment for measurement Stream switching and/or mixing allow generation of standards to match analytical requirements Stream switching and/or mixing allow generation of standards to match analytical requirements Reaction monitoring Microreactors and continuous flow reactors Batch reactors (with fast loop) Sample Preparation Gas handling (mixing, generation, delivery) Liquid handling (mixing, dilution, conditioning, etc.)

Special Lab NeSSI™ Requirements Flowing streams Need to move liquid and gas streams through NeSSI™ Most laboratory analysis involves discrete samples rather than sample streams Analytical characterization of flow in NeSSI™ needed Heating and Cooling Laboratory applications need both May require more rapid thermal response Integration with other lab apparatus Serial communication and legacy I/O Integration with lab software systems Low cost Competing with other solutions that don’t carry some of NeSSI’s more exotic features

NeSSI™ and Microreactors Investigate integration of NeSSI™ with continuous flow microreactors Initially as analyzer interface Eventually as complete fluidics framework Automation requires Gen II

Initial Sensor Integration Online Raman Probe Sample Flow Cell Continuous Microreactor Initial Sensor Integration

NeSSI Raman Sampling Block Parker Interflow NeSSI substrate Sample conditioning to induce backpressure to reduce bubble formation and the heated substrate allows analysis at reactor conditions

NESSI Microreactor Sampling/Calibration System Reactor Feed 1 Reactor Feed 2 Product Stream Real-time Calibration prod Raman Probe Pump 1 Pump 2 waste

Benefits of NeSSI™ to Reaction Analysis Conditioning and manipulation of sample introduced to analyzer Better control of sample physical parameters PhaseTemperatureVelocity Ability to implement sensors at points where measurement parameters are optimal Flexibility for performing calibration online without removing sensor Fast switching of streams for measurement, calibration or validation

NeSSI™ Fermentation System Switching filter system to handle particulate laden liquid streams Cells in fermentation broth Supplied by Bob Sherman, Circor Interested in plugging, cleaning, and other issues related to bio/pharma applications 2 Filters

Fermentation Headspace System Designed to allow fermentation headspace monitoring Fast circulation loop of headspace gases Addition of calibration gases Output to process Mass spectrometer Fermentor Headspace Loop Calibration gases To Mass Spec MFC

Example Sensor Interface Sensor is a vapochromatic compound Responds to different compounds by intensity and wavelength shifts in fluorescence signal Optical detection using simple VIS spectrometer LED excitation light source LED excitation light source Simple reflection optical fiber light path Simple reflection optical fiber light path Use of BallProbe to provide single-sided optical interface Vapochrome coated on ball surface NeSSI™ system to control delivery and mixing of gas stream

NeSSI Gas Mixing System Using a customized ballprobe reflectance probe for vapochromic detection - Ocean optics spectrometer - ballprobe coated with vapochrome - NeSSI substrate with 2 MFC’s

NeSSI Gas Mixing System Fluorescence response of vapochrome to dry N 2 and cylinder air Wavelength (nm) Intensity (counts) Dry N 2 Air Experiment - 25 ms - 20 accums Air Experiment - 25 ms - 20 accums

Vapochromic Response Movie

Vapochrome Summary Use of NeSSI™ system allowed for better control and characterization of sensor response Less variability and faster response to gas composition changes Ability to generate gas mixtures in situ Opens up potential for additional studies to quantitatively characterize a library of vapochromatic compounds to different analytes Investigate optimal sensor design

NeSSI™ Knowledge Network Main topic page divided into 4 sections News and Information Discussion (NeSSI Q&A) Component List Only Manufacturers group may post here Only Manufacturers group may post here Testing and Examples Main NeSSI™ web site NKN can be found at

Component List Sub-topics Page shown from the “Valve, Manual” topic Listing of topics under manual valve main topic Number of posts will correspond to different available components

Component List Page Example post from the component list page URL from Vendor Spec. sheet or link Picture, description, or link to more information Other Vendors are currently working to populate the list with more components

User Self-Registration Allows users to setup accounts Selecting to join “Manufacturers” group will queue user’s registration until a moderator approves it. Membership in other groups automatically approved immediately Profile data will be used for NeSSI™ mailing list Check the options available from the “Edit Profile” link once user activates account Once user submits registration form, they are sent an with instructions for activating their new account Your account is not active till you log in and follow the instructions in the

NKN Conclusion New software and web site ready to be used Need to work on additional instruction sheets Thanks to Michelle Cohn, UOP for helping CPAC buy a new server to host NeSSI™ and NKN web sites NeSSI Component List is becoming populated Major manufacturers already signed up and starting to supply content Need to get smaller suppliers to do the same Need to get user community to register and start using it Expect announcements soon (1-2 weeks)

NeSSI™ Sensor Survey The purpose of this survey is to gather information on the sensors and actuators needed for NeSSI Generation II Sample Systems, Analytical Systems, and Cluster Applications. The purpose of this survey is to gather information on the sensors and actuators needed for NeSSI Generation II Sample Systems, Analytical Systems, and Cluster Applications. This data will be shared with sensor and instrument vendors to help them prioritize development of the devices needed across industries and applications. This data will be shared with sensor and instrument vendors to help them prioritize development of the devices needed across industries and applications. The more information you can provide, the faster better products and performance can be delivered

NeSSI™ Sensor Survey (cont.) On-line survey form on the CPAC NeSSI™ web site NeSSI_Sensor_Survey.htm NeSSI_Sensor_Survey.htm Questions about condition sensors Are you interested How would you rank their importance Operating ranges Questions about analytical sensors Are you interested How would you rank their importance Operating ranges Questions about actuators Questions about “Clusters” Optional contact information

NeSSI™ Sensor Survey Results Condition Sensors Analytical Sensors

NeSSI™ Sensor Survey Results ActuatorsClusters 11 of the 15 respondents indicated they would share information with Vendors

NeSSI™ Sensor Survey (cont.) Plan is to continue to solicit responses from users Additions to survey questions or content welcome May expand survey activities to help provide market directions to suppliers Other aspects of NeSSI™ development Applet Software and other issues relate to SAM Applet Software and other issues relate to SAM New opportunities or innovations New opportunities or innovations Need to reach beyond the normal NeSSI™ enthusiasts Combined with the discussion board, surveys provide a valuable resource for both end-users and manufacturers of NeSSI

Summary & Conclusion NeSSI™ Gen II ready for development Working spec/prototype design complete Prototypes demonstrated in real applications Near resolution of the NeSSI™ bus issue For some laboratory applications Gen II and Gen III will immediately work and provide benefits The future holds a lot of promise!!