8.2 Structure of DNA KEY CONCEPT DNA structure is the same in all organisms.

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8.2 Structure of DNA KEY CONCEPT DNA structure is the same in all organisms.

8.2 Structure of DNA DNA is composed of four types of nucleotides. DNA is made up of a long chain of nucleotides. Each nucleotide has three parts. –a phosphate group –a deoxyribose sugar –a nitrogen-containing base phosphate group deoxyribose (sugar) nitrogen-containing base

8.2 Structure of DNA

The nitrogen containing bases are the only difference in the four nucleotides.

8.2 Structure of DNA T A C G Nucleotides always pair in the same way. The base-pairing rules show how nucleotides always pair up in DNA. Because a pyrimidine (single ring) pairs with a purine (double ring), the helix has a uniform width. – A pairs with T – C pairs with G

8.2 Structure of DNA Watson and Crick determined the three-dimensional structure of DNA by building models. They realized that DNA is a double helix that is made up of a sugar- phosphate backbone on the outside with bases on the inside.

8.2 Structure of DNA Watson and Crick’s discovery built on the work of Rosalind Franklin and Erwin Chargaff. –Franklin’s x-ray images suggested that DNA was a double helix of even width. –Chargaff’s rules stated that A=T and C=G.

8.2 Structure of DNA The backbone is made up of sugar and phosphate and connected by covalent bonds. hydrogen bond covalent bond The bases are connected by hydrogen bonds.

8.2 Structure of DNA Defined: Process of copying DNA –During interphase (S-stage) Step 1: DNA Helicase “unzips” the two DNA strands (breaks the H bonds) Step 2: Free floating nucleotides (A, T, C, G) match up Step 3: DNA Polymerase reconnects the two strands

8.2 Structure of DNA

There are many origins of replication in eukaryotic chromosomes. DNA replication starts at many points in eukaryotic chromosomes. Replication is fast and accurate. DNA polymerases can find and correct errors.

8.2 Structure of DNA Mutation: change in DNA sequence Mutations can be corrected –Enzyme “proofreads” DNA and check/correct errors Pre-repair: 1 in 10,000 nucleotides has an error Post-repair: 1 in 1 billion nucleotides has an error

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