Microscopy. I. Microscopes ENHANCES powers of observation; (LM, SEM, TEM). Microscopy and Measurement.

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Presentation transcript:

Microscopy

I. Microscopes ENHANCES powers of observation; (LM, SEM, TEM). Microscopy and Measurement

(1a) Magnification (ocular x objective = magnification) Increase of an object’s SIZE.

(1b) Field of View – size of the view of the object being looked at Decreases as magnification increases.

(2) Resolution Power to show magnified details CLEARLY.

(A) Light Microscope (10x to 400x, our scopes) Light REFLECTS off object and is MAGNIFIED through OBJECTIVE and OCULAR lenses.

(B) Electron Microscope (2,000x to 200,000x) Beam of e- used to PASS THROUGH (TEM) or BOUNCE OFF (SEM) a specimen.

(1) Why might a biologist PREFER to use the LOWER powers of a LIGHT microscope to OBSERVE aspects of unicellular organisms? Critical Thinking

(1) Transmission Electron Microscope (TEM) e- SHOT THROUGH specimen producing a 2-D image of HIGH magnification (must be DEAD).

(2) Scanning Electron Microscope (SEM) E- BOUNCE OFF outer surface  a 3-D image of specimen (also must be dead).

(2) Look at the photographs below. The SEM, left is of Staphylococcus aureus. The TEM, right, is also of S. aureus. Compare and contrast what EACH electron micrograph tells you about this organism. Critical Thinking

II. Measurement Standardized units (SI units) are used with universality (g, L, m). (A) Base Units and Derived Units Include length, mass, time, volume, and density.