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Chapter 4 Notes Part II: Microscopy (refer to pg.60-61)

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1 Chapter 4 Notes Part II: Microscopy (refer to pg.60-61)
Cells were discovered in the 17th century, when microscopes were invented….

2 Key term distinction Magnification: the amount of times a specimen being viewed is enlarged in order to be seen, varies with power of microscope Resolution: the ability that the microscope has to distinguish between two different objects, ie. The nucleus vs. the endoplasmic reticulum Contrast: ability to see detail within the specimen clearly

3 Compound light microscopes
What we work with in class Light rays pass through specimen and come into focus through two glass lenses. The eyepiece lens gives a total of 10x magnification. The objective lens varies with microscopes but can be up to 100x magnification. Total magnification is found by multiplying eyepiece lens by objective lens.

4 Assorted types of light microscopes:
Bright-field – the most common used in compound-light microscopes Phase contrast – microscope enhances density differences in specimen Differential interference – computer enhancement Darkfield – light passes through specimen at an angle, helpful in viewing organelles SEE PAGE 61!

5 Transmission Electron Microscopes
Use electrons to pass through specimens to view detail; image projected onto screen or photographic film

6 Scanning electron microscopes
The specimen is first coated with a thin layer of metal and an image is produced by the electrons which bounce off of the image

7 Immunofluorescence microscopy
Uses fluorescent antibodies to reveal proteins in cells Can look at specific proteins in detail

8 Confocal microscopy A major advancement in microscopic illumination
Uses a laser beam to focus on a shallow plane within cell which creates 3-D images

9 Today’s lab Microscope intro WS due next time!
READ directions and FOLLOW PROCEDURES!!!

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