1. Introduction to Microscopy: The Light Microscope The TEM The SEM

2. Introduction to Microscopy (Use of Microscopes to study minute [small] objects) light microscopeThe image to the right is a light microscope Light microscopes bounce light waves off of an object. The light source may be from the sun or from a bulb from a lamp. The diaphragm of a light microscope controls the amount of light the enters the stage to illuminate the specimen being examined.

3. Resolution: The resolution of a microscope is a measure of the clarity the image produced by the scope; the smallest distance between to points that can be seen as being separate. http://www.olympusmicro.com/primer/java/ microscopy/airydiscs/index.htmlhttp://www.olympusmicro.com/primer/java/ microscopy/airydiscs/index.html The image below shows the difference between the resolution of a light microscope versus an electron microscope

4. Electron Microscopes: these microscopes bounce a beam of electrons off of an object instead of light. These microscopes have a high resolution (smaller distance between two points)

5. There are two types of electron microscopes: SEM (scanning electron microscope) –Produces a three-dimensional image) TEM (transmission electron microscope) –Produces a two-dimensional image)

6. SEM Images (Three-Dimensional) http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html

7. TEM images

11. Which has better resolution of image?

12. Controls amount of light entering the stage

13. http://images.google.com/imgres?imgurl=http://shs.westport.k12.ct.us/mjvl/bi ology/microscope/parts.gif&imgrefurl=http://shs.westport.k12.ct.us/mjvl/biolog y/microscope/microscope.htm&h=314&w=458&sz=5&hl=en&start=1&um=1&t bnid=BLEnDPCBo0ZxeM:&tbnh=88&tbnw=128&prev=/images%3Fq%3Dho w%2Bto%2Buse%2Ba%2Bmicroscope%26svnum%3D10%26um%3D1%26h l%3Den