By:Jeibouei.Sh. STR s Molecular studies Parasites.

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Presentation transcript:

By:Jeibouei.Sh

STR s Molecular studies Parasites

Molecular epidemiology studies of E.histolytica and E.dispar Recent studies

 Genetic polymorphism of tRNA_linked STR_ containing locus A_L To clarify the genotypic differences among E.dispar isolates

 28 E.dispar strains 24 strains,Tehran and Zahedan Abdominal pain, Diarrhea, Bloating, Vomiting 4 strains, Gonbad Asymptomatic Direct smear Formalin_ether concentration Trichrom stain

DNA extraction from stool Locus A_L amplification by PCR Electrophoresis Sequencing the PCR products Edition and submittion to the Gen Bank

A genetic diversity in Size Number Position Of the repeat units. The variation of nucleotide sequence 448………472.……………..……..…507 E : The dominant genotype and The most frequent size

Discussion This study proposes that: isolates from a variety Analysis of genotypes of E.dispar isolates from a variety of locations help in determining the geografic origins of isolates and routes of transition Age, Sex, Parasite genotypeInfection with E.dispar Sequence type E Men more then Women Acymptomatic pationts more than the others

Thanks for your attention