1. Wednesday: Thursday: Experiments: 1: DNA-fingerprinting
2: Extraction of DNA
3: Microbiology: Assaying bacteria in our environment
Thursday:
Results from:
2: Microbiology: Assaying bacteria in our environment
4: Microbiology: Growth Factors and yeast
5: Blood: Testing for blood types

2. Location: Aalborghus Gymnasium, teachers’ preparation room
Crime Scene Report
Location: Aalborghus Gymnasium, teachers’ preparation room
Date: January the 31th, 07:30 AM

3. Primary suspects Suspect 1: Ole Suspect 3: Rolf Suspect 2: Thorbjørn
Suspect 4: Torben

5. Chromosomes and DNA

7. Variations i DNA SNP’s: Small Nuclear Polymorphisms
STR’s: Small Tandem Repeats
STR’s gives the opportunity to separate them by size

8. A person inherit a copy (allele) of a STR region from each of the parents HOMOZYGOTE: both alleles are of the same length (same number of repeats) HETEROZYGOTE: the alleles are of different length (unevenly number og repeats)

9. CODIS (FBI 1997) Combined DNA Index System

10. Procedure Get your samples (handed out to you)
Extract DNA (already extracted)
PCR: Multiply STR’s
Gel Electrophoresis: Separate STR’s
Stain DNA
View results –Compare suspects with Crime Scene DNA

11. Indsæt note og kildehenvisning via Header and Footer
PCR
You add:
Sample DNA
TAQ-polymerase
Primers 1 &2
nucleotides
Indsæt note og kildehenvisning via Header and Footer

12. Indsæt note og kildehenvisning via Header and Footer
Gel electroforesis
Indsæt note og kildehenvisning via Header and Footer