The Effects of Resveratrol and Alcohol on Short Term Memory in Drosophila Melanogaster By Paul Mascali

Need

Alcohol  A drug found through the fermentation of fruits  Alcohol deactivates the genes protein kinase and sirtuin 1

Resveratrol  Resveratrol is a chemical that is found in plants  Resveratrol activates the protein kinase and sirituin 1

Memory Perception

Alcohols Effect on the Nerve Alcohol effects the Dendrites on the ends of the Nerve

Wernicke-Korsakoff Syndrome results from severe acute deficiency of thiamine (Vitamin B1),

Resveratrol on lifespan in Drosophila and C. Elegans  Bass, 2007  Resveratrol was inserted in the drosophila food at 1, 3.2, 10, 32, 100, 200, and 1000 mM  Resveratrol was dissolved at a 1:1000 to molten agar NGM ratio during preparation of plates for C. Elegans

Drosophila Memory Notch is required for long-term memory in Drosophila Asaf Presente

Purpose The purpose of this experiment is to see how alcohol and resveratrol effects the short term memory in Drosophila Melanogaster

Hypothesis  Null Hypothesis: There will be no significant difference between the control group, the group exposed to alcohol, and the group exposed to resveratrol.  Alternative Hypothesis: The groups that are exposed to resveratrol will show more of an increase in short term memory than the control and the groups exposed to alcohol

Memory Testing Memory testing Memory learning

Exposure to Alcohol Testing Chamber Air Pump Ethanol Beaker

Exposure to Resveratrol

Methodology Wild Type Drosophila Control Group Never exposed to Resveratrol or ethanol N=200 Alcohol exposed Group Exposed to 200 Proof ethanol for 7 minutes N=200 Resveratrol exposed Group Exposed to 1000 Micro Liters of Resveratrol N=200 Resveratrol and Alcohol exposed Group Exposed to 200 proof ethanol for 7 minutes and 1000 Micro Liters of Resveratrol N=200 Flies put through shock training test Flies tested for memory Data put into SPSS for a one-way ANOVA and scheffe post-hoc test

Budget VendorCat.#PageDescribe ItemQuantityUnit PriceTotal Price CarolinaNp % Ethanol N/A Air pump1Already obtained N/A Drosophila Exposure Chamber1Already obtained N/A Ethanol beaker1Already obtained N/A Short term memory monitor 1 Already obtained N/A 4-methycyclihexanol 1 Already obtained

Do-ability Exposure to Resveratrol Exposure to Alcohol

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