AVEQ WP 8 Antioxidants in Oats Lena Dimberg and Rita Redaelli.

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AVEQ WP 8 Antioxidants in Oats Lena Dimberg and Rita Redaelli

Outline  Why antioxidants?  Antioxidants in oats  Tocopherols /tocotrienols/avenanthramides Bioactivities Effects of processing Effects of cultivation measures  Concluding remarks

Oxidation Oxidation of lipids generates free radicals which are very reactive –may results in reduction of shelf-life of foods –may cause damage to various body tissues, and may thereby initiate various diseases

Antioxidants Antioxidants effectively retard the onset of lipid oxidation with different mechanisms –may protect food from rancidity –may protect the body from free radical damages

Antioxidants  Ascorbic acid (Vitamin C)  Carotenoids (Vitamin A precursors)  Tocopherols/tocotrienols (Vitamin E)  Phenols

 Color  Flavor and aroma  Texture  Keeping qualities  Pharmacological effects Phenols in Foods

Tocopherols/ tocotrienols tocopherols tocotrienols IsomersR'R'' αCH3 β H γH δHH

Pisacane et al NP-HPLC OF TOCOLS

Preventive of foetuses' death Membrane stabilizer Tocopherols/tocotrienols -bioactivities- Antioxidants structure-activity relationship

Tocopherols- in germ Tocotrienols – in endosperm Genetic variability Tocotrienols are positively related to the oil content Tocopherols/tocotrienols

Range of variation in tocol content Content (mg/kg) Cultivar originAuthors USPeterson & Qureshi, high-oil linesPeterson & Wood, SwedishBryngelsson et al ItalianRedaelli et al. 2004

Avenanthramides R= H, OH or OCH 3 n= 1 or 2

Phytoalexin/Antifungal Antioxidative Structure- activity relationship Anti-atherosclerotic Anti-inflammatory Avenanthramides -bioactivities Cooling sensation Tranilast look alike

Mainly bran constituents Not related to the oil content Avenanthramides Unique to oats

Tranilast Metabolites Oat avenanthramide Tranilast

Supression of vascular stenosis by Tranilast Control Tranilast Fukuyama et. al European Journal of Pharmacology, 318,

Storage stable in whole grains Increase during germination Instable in raw oat flour soaked in water Avenanthramides -effects of processing- Heat stable

Effect of germination

Nitrogen/Light/Irrigation Conventional /Organic Farming Susceptible/Resistance Genotype/Site/Year Avenanthramides -effects of cultivation measures- Developmental stage

Avenanthramides - the same variety different year-

Concluding remarks Whole grain vs flour Processing Bioavalability Groats Flour