Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) Hypothesis Plasma biomarkers exist that.

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Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) Hypothesis Plasma biomarkers exist that can distinguish between DF and DHF early in disease progression In search of…. Biomarkers = Prognostic Tools = Understanding Disease Pathogenesis In search of Dengue Biomarkers Student: Maike-Svenja Milkereit - Supervisor: Dr. Brian Ward, Montréal General Hospital

Samples: –Disease (1o/2o, DF/DHF, time point collected, viral serotype) –Control Tools: –SELDI-TOF MS –CE & BPS Analysis –Biochemistry (Fractionations & Gels) Protein ID Biomarker Validation Experiments Investigating Biomarker Role in Pathogenesis Candidate Biomarkers In search of Dengue Biomarkers ?

Expected Results: –Candidate Biomarkers distinguishing DF/DHF/Control –Biomarker Identity: Host protein? Immunoregulatory? Affecting Vascular Endothelia? M/Z Signal Intensity SELDI-TOF MS Spectra of Dengue Patient Plasma Sample (#35, 1DHFt1) In search of Dengue Biomarkers Thank-you!