Analysis of amino acids and proteins

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Presentation transcript:

Analysis of amino acids and proteins Amino acids- ninhydrin and TLC Proteins- biuret test and electrophoresis

Mixture of aminoacids Separate by thin layer chromatography (TLC) Detect with ninhydrine (spray and heat) LILAC!

Mixture of proteins or aminiacids Separate with chromatography- electrophoresis Separation by molecular weight and charge PAGE- PolyAcrylamide Gel Electrophoresis

PAGE

Detection of proteins Detect with biuret test- add Cu2+ and NaOH, lilac coulour indicates protein. Complexes with the peptide bonds and forms a violet complex