Animal Behavior AP Biology Lab 11

Animal Behavior 2 Part Lab Part A: Response to Environmental Variables Activity A1: Initial Observation of Isopod Behavior Activity A2: Orientation Behavior of Isopods Activity A3: Designing an Experiment to Test Isopod Behavior Part B: Courtship Behavior in Fruit Fruit Flies

National Science Education Standards Unifying Concepts and Processes Systems, order, and organization Evidence, models, and explanation Constancy, change, and measurement Science as Inquiry Abilities necessary to do scientific inquiry Understandings about scientific inquiry Life Science Behavior of organisms

Objectives Observe animal behaviors relating to environmental stimuli Observe courtship behavior Design and conduct animal behavior experiments

Isopods Terrestrial Isopods Sow bugs (Porcellio laevis) and pill bugs (Armadillidium vulgare) Roly-polies, potato bugs, wood lice Breathe with gills, must remain moist Similar size, color, and life cycle Different Response to threats Pill bugs- curl up in tight ball for protection Sow bugs- either flee or remain perfectly still, appearing to be dead

Procedure- Activity 1A Observe the isopods for 10 minutes. Observe in their undisturbed behavior Note their behavior. Did you note any agonistic behavior (social interaction)? If so, explain the behavior. Are the movements of the isopods better described as taxis or kinesis movements? Taxis- an innate behavioral response by an organism to a directional stimulus or gradient of stimulus intensity EX. Chemotaxis, phototaxis, geotaxis, thermotaxis Kinesis- like taxis, a movement or activity of an organism to a stimulus. Different from taxis in that the response to the stimulus provided is non-directional

Procedure- Activity 2A Place clean filter paper into each side of the choice chamber. Using the dropping pipet, saturate the filter paper on one side of the chamber. Pour off any excess water; do not let the water run into the other side of the chamber or moisten the paper there. Using the sorting brush to transfer 5 isopods to each side of the chamber. Put on the lids. Count and record in Table 1 the number of animals on each side of the chamber, every 30 seconds for 10 minutes. Continue to record even if the isopods all move to one side or stop moving.

Procedure: Activity 3A Design an experiment to test isopod behavior Choice Chamber Side 1 Chamber Side 2 Cool vs. Warm Resealable plastic bag filled with ice Resealable plastic bag filled with warm water Light vs. Dark Exposed to light Wrapped with aluminum foil Light vs. Shade Covered with several screens Low pH vs. High pH A few drops of NaOH on filter paper A few drops of HCl on filter paper Substrate material vs. Absence of substrate material Substrate material present Substrate material absent