DEPARTMENT OF CELL BIOLOGY Head: Dr. Jacek Bigda, Associate Professor Research Staff: Dr. Małgorzata Doszczak Dr. Patrycja Koszałka Dr. Zuzanna Dobrzańska Ph.D. Students: Marcin Okrój Arkadiusz Pierzchalski

MAIN TOPIC Mechanism of action of tumor necrosis factor (TNF) in vitro & in vivo TEACHING cell biology, histology, immunology

DEPARTMENT OF CELL BIOLOGY research interests:  mechanism of IL-1-induced cytotoxicity;  intracellular signaling of tumor necrosis factor;  mechanism of toxicity of the anti-angiogenic fumagillin derivative, TNP-470;  mechanism of anti-tumor action of poly-RGD peptides;  mechanism of accelerated growth of hamster melanoma following LPS application.

U 937 – human histiocytic lymphoma TNF-R55 F N TF N TF N T TNF-R75 F N T m F N T m F N T

Cycloheximide (CHX) U937 cell variants

U 937 M U 937 G U 937 ATCC

IL-1 cytotoxicity

control 4 h 2 h 0,5 h 1 h TNF [pg/ml] control CHX IL-1 IL-1/CHX IL-1 cytotoxicity

Isotype control Untreated IL-1/CHX

DEPARTMENT OF CELL BIOLOGY financial support:  Analysis of mechanism regulating secretion of TNF following application of interleukin 1 and cycloheximide; State Committee for Scientific Research, Poland ( );  Mechanism of synergistic anti-tumor action of tumor necrosis factor (TNF) and angiogenesis inhibitor TNP-470; State Committee for Scientific Research, Poland ( );  Analysis of TNF sensitivity of variants of the U937 cell line; Intramural grant, Medical University of Gdańsk ( );  Modulation of tumor growth by means of cytokines; Core fund, Intercollegiate Faculty of Biotechnology UG-MUG;

DEPARTMENT OF CELL BIOLOGY selected previous grants:  Significance of tumor necrosis factor and its receptors for growth and dissemination of Bomirski melanoma; State Committee for Scientific Research, Poland ( );  Immunomodulatory and anti-tumor activity of cloned and purified rat tumor necrosis factor; State Committee for Scientific Research, Poland ( );  Influence of receptors of tumor necrosis factor for apoptosis of cancer cells; State Committee for Scientific Research, Poland ( );  Mechanism of cytotoxic action of interleukin 1; State Committee for Scientific Research, Poland ( );  TNF gene polymorphism and humoral reactivity against p53 protein in patients suffering from non-small cell lung cancer; State Committee for Scientific Research, Poland( ).

Tri-City Central Animal Laboratory, Medical University of Gdańsk

Tri-CityCentral Animal Laboratory, Medical University of Gdańsk Tri-City Central Animal Laboratory, Medical University of Gdańsk