ELF-Induced DNA Breaks

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Presentation transcript:

ELF-Induced DNA Breaks Henry Lai Department of Bioengineering University of Washington Seattle, WA USA

DNA Strand breaks -single and double strand breaks -mis-repair cumulative -affect cell functions; can lead to cancer and cell death

COMET ASSAY

DNA strand breaks have been reported in different cell types after ELF EMF exposure. Ahuja (99)- human leukocytes Delimaris (06)- human lymphocytes Hong (05)- mouse sperm cells Ivancsit (02, 05)- human fibroblasts and melanocytes Ivancsit (03)-more pronounced effect observed in fibroblasts of older subjects. Jajte (01)- rat lymphocytes Lai and Singh (97, 04)- rat brain cells Li (01)- E. coli Svedenstal (99)- mouse brain cells Wolf (05)- HL-60 and rat fibroblasts Zmyslong (00, 04)- rat lymphocytes

ELF EMF affects DNA repair Robison (02) HL-60 –decrease Chow (00) – E. coli- increase- heat shock protein Schmitz (04)- Mouse brain and kidney cells- increase

ELF EMF genotoxic effects involve free radicals Effects blocked by free radical scavengers, enhanced by divalent metals, increase oxidative-damage in molecules Jaite (01) Lai and Singh (97) Li (01) Lourencini (00) Yokus (05) Wolf (05) Zmyslong (00, 04)

THE FENTON REACTION Electromagnetic fields iron OH H2O2 mitochondria Cellular damage THE FENTON REACTION

Intensity and duration of exposure interact. High intensity/short duration = Low intensity/long duration