A) What is DNA Barcoding i) What are the barcoding regions and what are their properties that make them useful? [Richard Pyle, Bishop Museum, USA – amongst others]

A) What is DNA Barcoding ii) Would the DNA barcode represent a *definition* of a species? [David Fitch, New York University, USA – amongst others]

A) What is DNA Barcoding iii) How would DNA barcoding work in practice and who should be doing it? [Vince Smith, Illinois Natural History Survey, USA]

B) DNA barcoding in species identification i) How will the DNA barcoding identifications deal with the overlap between intraspecific and interspecific variation documented in many groups? [Everybody!]

B) DNA barcoding in species identification ii) To what extent will DNA barcoding support the efforts of traditional taxonomy? [David Yeates, CSIRO, Australia]

B) DNA barcoding in species identification iii) Is accuracy of identification (to the right clade) possible in the absence of accuracy in family- and genus- level topology? [Jim Hayden, Cornell University, USA]

C) DNA barcoding in species discovery i) How confident can we be in the utility of DNA barcodes to discover new species when it has been demonstrated that many species are not mitochondrially monophyletic, and thus share mitochondrial polymorphisms with other species? [Daniel Funk, Vanderbilt University, USA]

C) DNA barcoding in species discovery ii) With a program of DNA barcoding what would be sufficient to demonstrate that a specimen represents a new species? [Kevin Johnson, Illinois Natural History Survey, USA]

D) Political & practical implications i) What will be the role of systematists in a world where most identifications are done by "barcode" and will the expansion of sequencing efforts come at the expense of systematics in general [Jim Hayden, Cornell University, USA]

D) Political & practical implications ii) Assuming the technical problems of DNA barcoding can be overcome is it now, or will it ever be cost-effective relative to traditional methods to use DNA barcodes for bioinventory purposes? [Derek S. Sikes, University of Calgary, Canada]

D) Political & practical implications iii) Will identification by barcodes increase people's enthusiasm for living things? [Jim Hayden, Cornell University, USA]