Network Services for Biologists in the Genome Era The Work of the European Bioinformatics Institute

Our Genome tcaattctga tcgaataaac gaatttacat atttggtaag ttttggccaa tttcgtagca 60 atatgatgaa attgcgctct tttttaggaa tatcaaattg gaatataaca aaaaaaaaac 120 tgaaactaac caactgaatc taatgtgcat tttaaataat aaaaatggat cattttatac 180 atcatattaa aattaaaaaa atttcataaa aataatacgt agtaaaaaat aaaaattttt 240 aacataaata aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEDP NAGDGEPKEQ IQDVEDQDVS Chr basepairs estimated 50, ,000 genes (3286 Mbases) 2/3 of which are completed and in the public domain.

...others... From: Genome MOT at the EBI (April 2000)

Data growth (EMBL DB)

Activity Areas at EBI EMBL –Archiving, development and distribution of DNA sequence data. Swiss-Prot –Archiving, production, development and distribution of Protein sequence data. MSD –Archiving and distribution of macromolecular structural data and structure prediction applications. DALI –Archiving and distribution of 2D/3D prediction databases and tools for their usage. ENSEMBL –Archiving, automatic analysis and distribution of Human genome data. CGG –Genome annotation, data mining, methabolic pathways research. CORBA –Design and implementation of CORBA-based tools for database querying SRS –Development and maintenance of SRS in collab.with Lionbiosciences. Industry –Links to industry and customised R&D (e.g. Gene Expression). External Services –Development and deployment of on-line interactive and non-interactive tools for sequence analysis.

EBI’s Network Services

Our common user interface

srs.ebi.ac.uk Sequence Retrieval System Core text search and retrieval engine for most services offered from EBI. Updates and links together more than 100 databanks. Biggest SRS server in the world (over 130 databases).

Genome & Proteomes Currently more that 30 complete genomes and proteomes are available interactively to the user community and demand for data from the Human genome is being met by providing access the all the material available in the EBI databases.

GPCRDB

A recent initiative: Ensembl Bringing discovery to the scientific community...

The Community EMBnet - European Molecular Biology network. Formed (officially) in 1988 to disseminate up-to-date molecular biology databases within member states. The initiative for the creation of EMBnet was started by EMBO council members in collaboration with EMBL staff in 1986.

Dissemination of EBI data resources to the world through the EMBnet

An EMBnet node (1/2) Hosted by a national academic centre. Has national coverage over the Internet. Provides services to academics as well as industry (ca users per node). Maintains local copies of the mayor biological databases and sequence analysis packages.

An EMBnet node (2/2) Provides training and education in the national language. Each node typically employs 2-3 staff. Each node has at least one major interactive login server and a WWW and ftp server (ca. 300 hosts today).

EMBnet organisation and main tasks ano 2K

EMBnet membership

EMBnet Milestones (1/2) Development of network based tools for database updates: – First transaction of sequence data between EMBL in Heidelberg and InfoBiogen in Paris. – First implementation of the HASSLE protocol between Norway, Switzerland and Italy. Asynchronous sequence database updates where then possible. – First implementation of xNDT between Norway and Sweden. Asynchronous sequence database updates…another solution. – First implementation of SynChron which runs from EBI today on several industrial sites.

EMBnet Milestones (2/2) Financial – The European Union grants support for EMBnet for the first time. Organisation – The Stichting EMBnet is formed granting EMBnet independence from any mayor member (e.g. EMBL). Software – SRS development was partly financed by EMBnet. – EMBOSS (under a GPL) is developed by EMBnet members.

Latest News New MPsrch - Fastest Smith & Waterman searches in the world (1.6 billion cell updates/sec) …available soon. Ensembl - fast delivery of newly predicted human genes and gene products into the public domain and access to similarity and homology searches on up-to- date data sets. Pre-calculated proteomic comparisons of genomes through InterPro. EST clustering, clean-up and redundancy reduction via the EuroGene Index.

Some facts and figures…(1/2) EMBL-EBI is the main provider of biology related sequence databases in Europe. –Sequence Databases (EMBL, TrEMBL, Ensembl (The Human Genome), etc.) –Cartographic Databases (RHdb) –Mutation Databases (HGBASE) –3D/2D Structure Databases (PDB, DSSP, etc.)

Some facts and figures….(2/2) EMBL-EBI produces more than 50 biological databases. EMBL-EBI handles ca. 100K request/day on and 170K requests/day on srs.ebi.ac.uk. (8M req./month) increasing at a rate of 15%/month. EMBL-EBI is moving more than 200Gb of data across the European networks each month.

Main usage is... Sequence querying and retrieval. Sequence comparison and searching. File distribution through ftp.ebi.ac.uk. Replication of data at many international sites (e.g. EMBnet nodes). Systematic use of based services.

Contacts EMBnet: EBI: corba.ebi.ac.uk, msd.ebi.ac.uk, fly.ebi.ac.uk, industry.ebi.ac.uk, interpro.ebi.ac.uk, etc. Ensembl: EMBL: