Living Organisms Consist of Cells State the resolution and magnification that can be achieved by a light microscope Explain the difference between magnification.

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Presentation transcript:

Living Organisms Consist of Cells State the resolution and magnification that can be achieved by a light microscope Explain the difference between magnification and resolution Explain the need for staining samples in light microscopy

Resolution and Magnification Car Headlights from far awayCar Headlights Close-Up

Define the Following Terms Magnification Resolution

Light Microscope Number of lenses used to produce an image Light passes from the bulb (or mirror) under the stage through a condenser lens and then through the specimen The light beam is focused through the objective lens then the eyepiece lens

How do you calculate the magnification power of a light microscope? Eyepiece lens magnification x Objective lens magnification The max magnification of a light microscope is x1500 The max resolution of a light microscope is 200nm

An Electron Microscope

Chloroplasts Electron micrograph Leaf cells in moss ; viewed using light Microscope; chloroplasts visible, but not detailed

Optical microscope image of blood cell smear.

SEM image of red blood cells.

TEM image of a thin section of a blood vessel. A white blood cell is entering the tissue between two epithelial cells.