Vrn1_T4 (AS_ITA_21_HQ910536) genomic sequence vs. corresponding fragment from T. aestivum Vrn-A1: F primer Exon 3Intron 3 Exon 4 Intron 4 Supplement 4:

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Vrn1_T4 (AS_ITA_21_HQ910536) genomic sequence vs. corresponding fragment from T. aestivum Vrn-A1: F primer Exon 3Intron 3 Exon 4 Intron 4 Supplement 4: alignments for putative Vrn1 clone used for RFLP mapping (November, 2011):

Intron 4Exon 5 Intron 5 R primer Intron 5 Exon 6 79% identity

AS_ITA_21_HQ spliced sequence* vs. A. strigosa Fruitful-like cDNA and spliced T. aestivum Vrn-A1 sequence: * Two sequencing errors corrected to match oat Vrn1 consensus sequence

AS_ITA_21_HQ translated sequence vs. Z. mays TF15 protein sequence and translated A. strigosa Fruitful-like and T. aestivum Vrn-A1 sequences: K-box motif