Objective Computer Assisted Review of DNA Evidence Dan E. Krane Wright State University, Dayton, OH 45435

What you see when you open a CD provided in discovery:

What you see when you try to open the files on a discovery CD:

An electropherogram using the softwares default settings: GenoTyper Graph

What the testing lab provides is not always the same as the default electropherogram:

Genophiler Zoom Genophiler also provides a zoomed graph to 150 RFUs Shows smaller peaks more clearly Here, the peaks that were disregarded by the lab may be evidence of an unknown, minor contributor to the sample

No mention of a potential, secondary contributor to the sample.

Still no mention of a potential, secondary contributor to the sock sample.

Genophiler output: Testing labs report:

Genophiler also flags potential problems for further review:

Project Screen Button Screen shot of GeneScan Shows exactly what samples were added and what parameter files were used for the analysis

Analysis Parameters Buttons See exactly what analysis settings were used Parameter files also copied to CD

Finding Problems With Samples: Raw Data Good raw data begins with a large peak followed by smaller peaks

Finding Problems With Samples: Raw Data Bad raw data is indicated by wildly uneven peaks Indicates lab analysis problems

Finding Problems With Samples: EPT Data Shows current, voltage, laser power, and temperature For good analysis, should be constant (flat)

Finding Problems With Samples: EPT Data Problem EPT data is not flat Indicates problems with lab analysis

Automation Process Select samples and settings in one screen Analysis can be performed unattended Up to 4 analysis runs can be set up at one time

Forensic BioInformatic Services Uses Genophiler to generate easily interpreted files Objectively applies analysis parameters to all samples Fast turn around times Efficiently draws attention to problems requiring further review

Acknowledgements: Genophiler programmers: Dr. Travis Doom Dr. Mike Raymer Dr. Oscar Garcia Jason R. Gilder STR experts: Dr. Bill Thompson Dr. Simon Ford Dr. Dan Krane Support: Wright State University, Dayton, OH Forensic BioInformatic Services; Suite 150, 2850 Presidential Drive, Fairborn, OH, 4538x; phone: (937) xxx-xxxx; web site: BioForensics.com