1. FSS-i 3 DNA expert system suite

2. DNA Profiling Sample e.g. hair, skin

3. DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data

4. DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data Produce a profile of the DNA

5. DNA Profiling Sample e.g. hair, skin Process sample to obtain raw data Produce a profile of the DNA Store profile in NDIS (National DNA Index System) or match to existing profile

6. Expert System profiling motivation Sample & data processing already heavily automated

7. Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck.

8. Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS

9. Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS Save enormous amounts of review time

10. FSS-i 3 Expert System FSS: Forensic Science Service of the UK

11. FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation

12. FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists

13. FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community –USA, Canada, Europe, Australia, New Zealand

14. FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 –6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community –USA, Canada, Europe, Australia, New Zealand Full Audit system

15. i-STRess The core DNA interpretation tool

16. i-STRess The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters

17. i-STRess The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters Detect multi-person DNA mixtures within a profile & export to i-STReam…

18. i-STReam Unique functionality to resolve two person DNA mixtures

19. i-STReam Unique functionality to resolve two person DNA mixtures Allows previously unusable DNA profiles to be uploaded to DNA databases

20. i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture

21. i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review

22. i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review Saves time: no more manual reviews of profiles

23. FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing

24. FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing 1 million+ profiles uploaded to UK National DNA DB

25. FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems –6 years of testing 1 million+ profiles uploaded to UK National DNA DB FSS-i 3 validation project: –70 000 previously analyzed samples –Minor discrepancies in 0.03% of samples & each of these samples were flagged by the system for further review

26. Technology used in FSS-i 3 Strategies for implementing rules: unknown

27. Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings

28. Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings Designed to run on stand-alone Pentium 3 PCs

29. Design & Development of FSS-i 3 Development time: six years

30. Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1)

31. Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1) i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton (1)

32. Conclusions: FSS-i 3 Consistent profiling of samples

33. Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles

34. Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination

35. Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination Significant reduction in time spent on the above, due to automation

36. References (1) http://www.forensic.gov.uk/forensic_t/i3/index.htmhttp://www.forensic.gov.uk/forensic_t/i3/index.htm (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdfhttp://www.promega.com/profiles/802/profilesindna_802_08.pdf (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpe rtSys.pdf http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpe rtSys.pdf (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdfhttp://www.promega.com/profiles/1001/profilesindna_1001_11.pdf (5) http://www.kathyreichs.com/dnaprofiling.htmhttp://www.kathyreichs.com/dnaprofiling.htm (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfmhttp://www.biotechnologyonline.gov.au/human/dnaprofile.cfm