BIOLOGY 2250 MICROBIAL GROWTH and ITS CONTROL. BIOLOGY 2250 PART 1. MICROBIAL GROWTH.

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BIOLOGY 2250 MICROBIAL GROWTH and ITS CONTROL

BIOLOGY 2250 PART 1. MICROBIAL GROWTH

Requirements for Growth Physical Requirements – Temperature, pH, Osmotic Pressure Chemical Requirements – Carbon, Nitrogen, Sulfur, Phosphorous, Trace Elements, Oxygen, Organic Growth Factors

Temperature Requirements Mesophiles Psychrophiles Psychotrophs Thermophiles Hyperthermophiles

TEMPERATURE OF FOODS

OSMOTIC PRESSURE

OXYGEN REQUIREMENTS

BINARY FISSION Little increase in size Growth in numbers to form colonies on an agar plate

How about these numbers!

BACTERIAL GROWTH

GROWTH PHASES

BIOLOGY 2250 PART 2. CONTROL OF MICROBES

TERMINOLOGY

MICROBIAL DEATH

Types of Cell Damage Alterations of Plasma Membrane Damage to Proteins and Nucleic Acids

PHYSICAL METHODS OF CONTROL Moist heat Dry heat Filtration High pressure Desiccation Osmotic pressure radiation

Moist Heat Autoclave

Principle of Autoclave Adjust your recipe to your altitude!

Dry Heat Oven Bunsen burner flame

FILTRATION Membrane filters have different pore sizes, e.g µ’s

High Pressures Fruit Juices

Desicattion Freeze Dried Foods Dried Meats Dried Fruits

Osmotic Pressure Salting – Pickling -

RADIATION Ionizing – cobalt, gamma rays Nonionizing – UV light Microwave

CHEMICAL CONTROL

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